Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/16166
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dc.titleGenetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
dc.contributor.authorYANG YI
dc.date.accessioned2010-04-08T11:01:46Z
dc.date.available2010-04-08T11:01:46Z
dc.date.issued2007-05-19
dc.identifier.citationYANG YI (2007-05-19). Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/16166
dc.description.abstractDevelopment of effective and safe gene delivery vectors is one of the most critical and challenging parts in gene therapy applications. In this study, we focused on the improvement of gene delivery efficiency of a newly emerged viral vector originated from insect viruses, namely baculovirus. Several important technical issues related to baculovirus vector production and purification processes were investigated. Through genetic modifications of Baculovirus, neuron-specific transgene expression was successfully achieved after local injection of the viral vector into the rat brain. A surface modification technology based on electrostatic interactions was developed to protect the envelope of the virus with a cationic polymer. The modified viral vectors were more resistant to serum inactivation and displayed an improved efficiency of transgene expression after systemic injection of the baculovirus vectors. With further characterization and modification, these viral gene delivery systems should have great potential in clinical central nervous system gene therapy.
dc.language.isoen
dc.subjectGene delivery, Baculovirus, virus surface modification, promoter engineering, central nervous system, glioma
dc.typeThesis
dc.contributor.departmentGRADUATE PROGRAMME IN BIOENGINEERING-SOM
dc.contributor.supervisorWANG SHU
dc.contributor.supervisorFENG SI-SHEN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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