Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0172131
Title: MicroRNAs regulating cluster of differentiation 46 (CD46) in cardioembolic and non-cardioembolic stroke
Authors: Tan J.R.
Tan K.S.
Yong F.L.
Armugam A.
Wang C.W.
Jeyaseelan K.
Wong P.T.-H. 
Keywords: membrane cofactor protein
messenger RNA
microRNA
microRNA 185
microRNA 19a
microRNA 20a
microRNA 374b
unclassified drug
3' untranslated region
membrane cofactor protein
microRNA
3' untranslated region
adult
aged
Article
bioinformatics
cardioembolic stroke
CD46 gene
cerebrovascular accident
clinical article
controlled study
disease classification
down regulation
female
gene expression regulation
gene ontology
human
human cell
luciferase assay
male
middle aged
non cardioembolic stroke
protein expression
receiver operating characteristic
sensitivity and specificity
signal transduction
umbilical vein endothelial cell
upregulation
area under the curve
case control study
cerebrovascular accident
chemistry
genetics
metabolism
nucleotide sequence
pathology
reporter gene
sequence alignment
very elderly
3' Untranslated Regions
Adult
Aged
Aged, 80 and over
Antigens, CD46
Area Under Curve
Base Sequence
Case-Control Studies
Female
Genes, Reporter
Human Umbilical Vein Endothelial Cells
Humans
Male
MicroRNAs
Middle Aged
ROC Curve
Sequence Alignment
Stroke
Issue Date: 2017
Citation: Tan J.R., Tan K.S., Yong F.L., Armugam A., Wang C.W., Jeyaseelan K., Wong P.T.-H. (2017). MicroRNAs regulating cluster of differentiation 46 (CD46) in cardioembolic and non-cardioembolic stroke. PLoS ONE 12 (2) : e0172131. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0172131
Rights: Attribution 4.0 International
Abstract: Ischemic stroke is a major cause of mortality and morbidity globally. Among the ischemic stroke subtypes, cardioembolic stroke is with poor functional outcome (Modified Rankin score ? 2). Early diagnosis of cardioembolic stroke will prove beneficial. This study examined the microRNAs targeting cluster of differentiation 46 (CD46), a potential biomarker for cardioembolic stroke. CD46 mRNA level was shown to be differentially expressed (p < 0.001) between cardioembolic stroke (median = 1.32) and non-cardioembolic stroke subtypes (large artery stroke median = 5.05; small vessel stroke median = 6.45). Bioinformatic search showed that miR-19a, -20a, -185 and -374b were found to target CD46 mRNA and further verified by luciferase reporter assay. The levels of miRNAs targeting CD46 were significantly reduced (p < 0.05) in non-cardioembolic stroke patients (large artery stroke median: miR-19a = 0.63, miR-20a = 0.42, miR-185 = 0.32, miR-374b = 0.27; small artery stroke median: miR-19a = 0.07, miR-20a = 0.06, miR-185 = 0.07, miR-374b = 0.05) as compared to cardioembolic stroke patients (median: miR-19a = 2.69, miR-20a = 1.36, miR-185 = 1.05, miR-374b = 1.23). ROC curve showed that the miRNAs could distinguish cardioembolic stroke from non-cardioembolic stroke with better AUC value as compared to CD46. Endogenous expression of CD46 in Human Umbilical Vein Endothelial Cells (HUVECs) were found to be regulated by miR-19a and miR-20a. Thus implicating that miR-19a and -20a may play a role in pathogenesis of cardioembolic stroke, possibly via the endothelial cells. © 2017 Tan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161530
ISSN: 19326203
DOI: 10.1371/journal.pone.0172131
Rights: Attribution 4.0 International
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