Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0129825
Title: Denovo generated human red blood cells in humanized mice support Plasmodium falciparum infection
Authors: Amaladoss A.
Chen Q. 
Liu M.
Dummler S.K.
Dao M.
Suresh S.
Chen J.
Preiser P.R.
Keywords: clodronic acid
erythropoietin
granulocyte antibody
interleukin 3
liposome
plasmid DNA
animal experiment
animal model
Article
B lymphocyte
blood sampling
controlled study
erythrocyte
ex vivo study
female
hematopoietic stem cell
human
human cell
immune system
immunocompetent cell
immunofluorescence
malaria falciparum
male
microscopy
mouse
newborn
nonhuman
polymerase chain reaction
T lymphocyte
animal
blood
chimera
cytology
disease model
erythrocyte
malaria falciparum
metabolism
nonobese diabetic mouse
parasitology
pathogenicity
pathology
Plasmodium falciparum
SCID mouse
Mus
Plasmodium falciparum
Animals
Chimera
Disease Models, Animal
Erythrocytes
Female
Humans
Malaria, Falciparum
Male
Mice
Mice, Inbred NOD
Mice, SCID
Plasmodium falciparum
Issue Date: 2015
Citation: Amaladoss A., Chen Q., Liu M., Dummler S.K., Dao M., Suresh S., Chen J., Preiser P.R. (2015). Denovo generated human red blood cells in humanized mice support Plasmodium falciparum infection. PLoS ONE 10 (6) : e0129825. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0129825
Rights: Attribution 4.0 International
Abstract: Immunodeficient mouse-human chimeras provide a powerful approach to study host specific pathogens like Plasmodium (P.) falciparum that causes human malaria. Existing mouse models of P. falciparum infection require repeated injections of human red blood cells (RBCs). In addition, clodronate lipsomes and anti-neutrophil antibodies are injected to suppress the clearance of human RBCs by the residual immune system of the immunodeficient mice. Engraftment of NOD-scid Il2rg-/- mice with human hematopoietic stem cells leads to reconstitution of human immune cells. Although human B cell reconstitution is robust and T cell reconstitution is reasonable in the recipient mice, human RBC reconstitution is generally poor or undetectable. The poor reconstitution is mainly the result of a deficiency of appropriate human cytokines that are necessary for the development and maintenance of these cell lineages. Delivery of plasmid DNA encoding human erythropoietin and interleukin-3 into humanized mice by hydrodynamic tail-vein injection resulted in significantly enhanced reconstitution of erythrocytes. With this improved humanized mouse, here we show that P. falciparum infects de novo generated human RBCs, develops into schizonts and causes successive reinvasion. We also show that different parasite strains exhibit variation in their ability to infect these humanized mice. Parasites could be detected by nested PCR in the blood samples of humanized mice infected with P. falciparum K1 and HB3 strains for 3 cycles, whereas in other strains such as 3D7, DD2, 7G8, FCR3 and W2mef parasites could only be detected for 1 cycle. In vivo adaptation of K1 strain further improves the infection efficiency and parasites can be detected by microscopy for 3 cycles. The parasitemia ranges between 0.13 and 0.25% at the first cycle of infection, falls between 0.08 and 0.15% at the second cycle, and drops to barely detectable levels at the third cycle of infection. Compared to existing mouse models, our model generates human RBCs de novo and does not require the treatment of mice with immunomodulators. Copyright: © 2015 Amaladoss et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161504
ISSN: 19326203
DOI: 10.1371/journal.pone.0129825
Rights: Attribution 4.0 International
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