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https://doi.org/10.1371/journal.pone.0133899
Title: | Metabolic plasticity and inter- compartmental interactions in rice metabolism: An analysis from reaction deletion study | Authors: | Shaw R. Kundu S. |
Keywords: | adenosine triphosphate nicotinamide adenine dinucleotide phosphate biomass chloroplast cytology cytosol genetics genotype metabolic flux analysis metabolism mitochondrion Oryza photon photosynthesis radiation response Adenosine Triphosphate Biomass Chloroplasts Cytosol Genotype Metabolic Flux Analysis Metabolic Networks and Pathways Mitochondria NADP Oryza Photons Photosynthesis |
Issue Date: | 2015 | Citation: | Shaw R., Kundu S. (2015). Metabolic plasticity and inter- compartmental interactions in rice metabolism: An analysis from reaction deletion study. PLoS ONE 10 (7) : e0133899. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0133899 | Rights: | Attribution 4.0 International | Abstract: | More than 20% of the total caloric intake of human population comes from rice. The expression of rice genes and hence, the concentration of enzymatic proteins might vary due to several biotic and abiotic stresses. It in turn, can influence the overall metabolism and survivability of rice plant. Thus, understanding the rice cellular metabolism, its plasticity and potential readjustments under different perturbations can help rice biotechnologists to design efficient rice cultivars. Here, using the flux balance analysis (FBA) method, with the help of in-silico reaction deletion strategy, we study the metabolic plasticity of genomescale metabolic model of rice leaf. A set of 131 reactions, essential for the production of primary biomass precursors is identified; deletion of any of them can inhibit the overall biomass production. Usability Index (IU) for the rest of the reactions are estimated and based on this parameter, they are classified into three categories-maximally-favourable, quasifavourable and unfavourable for the primary biomass production. The lower value of 1 - IU of a reaction suggests that the cell cannot easily bypass it for biomass production. While some of the alternative paths are energetically equally efficient, others demand for higher photon. The variations in (i) ATP/NADPH ratio, (ii) exchange of metabolites through chloroplastic transporters and (iii) total biomass production are also presented here. Mutual metabolic dependencies of different cellular compartments are also demonstrated. © 2015 Shaw, Kundu. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161495 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0133899 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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