Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0133899
Title: Metabolic plasticity and inter- compartmental interactions in rice metabolism: An analysis from reaction deletion study
Authors: Shaw R. 
Kundu S.
Keywords: adenosine triphosphate
nicotinamide adenine dinucleotide phosphate
biomass
chloroplast
cytology
cytosol
genetics
genotype
metabolic flux analysis
metabolism
mitochondrion
Oryza
photon
photosynthesis
radiation response
Adenosine Triphosphate
Biomass
Chloroplasts
Cytosol
Genotype
Metabolic Flux Analysis
Metabolic Networks and Pathways
Mitochondria
NADP
Oryza
Photons
Photosynthesis
Issue Date: 2015
Citation: Shaw R., Kundu S. (2015). Metabolic plasticity and inter- compartmental interactions in rice metabolism: An analysis from reaction deletion study. PLoS ONE 10 (7) : e0133899. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0133899
Rights: Attribution 4.0 International
Abstract: More than 20% of the total caloric intake of human population comes from rice. The expression of rice genes and hence, the concentration of enzymatic proteins might vary due to several biotic and abiotic stresses. It in turn, can influence the overall metabolism and survivability of rice plant. Thus, understanding the rice cellular metabolism, its plasticity and potential readjustments under different perturbations can help rice biotechnologists to design efficient rice cultivars. Here, using the flux balance analysis (FBA) method, with the help of in-silico reaction deletion strategy, we study the metabolic plasticity of genomescale metabolic model of rice leaf. A set of 131 reactions, essential for the production of primary biomass precursors is identified; deletion of any of them can inhibit the overall biomass production. Usability Index (IU) for the rest of the reactions are estimated and based on this parameter, they are classified into three categories-maximally-favourable, quasifavourable and unfavourable for the primary biomass production. The lower value of 1 - IU of a reaction suggests that the cell cannot easily bypass it for biomass production. While some of the alternative paths are energetically equally efficient, others demand for higher photon. The variations in (i) ATP/NADPH ratio, (ii) exchange of metabolites through chloroplastic transporters and (iii) total biomass production are also presented here. Mutual metabolic dependencies of different cellular compartments are also demonstrated. © 2015 Shaw, Kundu.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161495
ISSN: 19326203
DOI: 10.1371/journal.pone.0133899
Rights: Attribution 4.0 International
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