Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0089124
Title: O-hexadecyl-dextran entrapped berberine nanoparticles abrogate high glucose stress induced apoptosis in primary rat hepatocytes
Authors: Kapoor R.
Singh S.
Tripathi M. 
Bhatnagar P.
Kakkar P.
Gupta K.C.
Keywords: berberine
caspase 3
caspase 9
dextran
glucose
nanoparticle
o hexadecyl dextran
phosphatidylserine
protein Bax
protein bcl 2
unclassified drug
berberine
dextran
DNA
glucose
glutathione
nitric oxide
O-hexadecyl-dextran
protein bcl 2
reactive oxygen metabolite
superoxide dismutase
animal cell
antioxidant activity
apoptosis
article
cell cycle G1 phase
cell membrane depolarization
cell protection
cell stress
cell viability
concentration (parameters)
controlled study
drug efficacy
enzyme activation
glucose stress
lipid peroxidation
liver cell
male
mitochondrial membrane potential
nonhuman
oxidative stress
rat
animal
apoptosis
chemistry
dose response
drug effects
metabolism
mitochondrion
oxidative stress
particle size
secretion (process)
transport at the cellular level
Animals
Apoptosis
Berberine
Biological Transport
Cytoprotection
Dextrans
DNA
Dose-Response Relationship, Drug
Glucose
Glutathione
Lipid Peroxidation
Male
Mitochondria
Nanoparticles
Nitric Oxide
Oxidative Stress
Particle Size
Proto-Oncogene Proteins c-bcl-2
Rats
Reactive Oxygen Species
Superoxide Dismutase
Issue Date: 2014
Citation: Kapoor R., Singh S., Tripathi M., Bhatnagar P., Kakkar P., Gupta K.C. (2014). O-hexadecyl-dextran entrapped berberine nanoparticles abrogate high glucose stress induced apoptosis in primary rat hepatocytes. PLoS ONE 9 (2) : e89124. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0089124
Rights: Attribution 4.0 International
Abstract: Nanotized phytochemicals are being explored by researchers for promoting their uptake and effectiveness at lower concentrations. In this study, O-hexadecyl-dextran entrapped berberine chloride nanoparticles (BC-HDD NPs) were prepared, and evaluated for their cytoprotective efficacy in high glucose stressed primary hepatocytes and the results obtained compared with bulk berberine chloride (BBR) treatment. The nanotized formulation treated primary hepatocytes that were exposed to high glucose (40 mM), showed increased viability compared to the bulk BBR treated cells. BC-HDD NPs reduced the ROS generation by ~3.5 fold during co-treatment, prevented GSH depletion by ~1.6 fold, reduced NO formation by ~5 fold and significantly prevented decline in SOD activity in stressed cells. Lipid peroxidation was also prevented by ~1.9 fold in the presence of these NPs confirming the antioxidant capacity of the formulation. High glucose stress increased Bax/Bcl2 ratio followed by mitochondrial depolarization and activation of caspase-9/-3 confirming involvement of mitochondrial pathway of apoptosis in the exposed cells. Co- and post-treatment of BC-HDD NPs prevented depolarization of mitochondrial membrane, reduced Bax/Bcl2 ratio and prevented externalization of phosphatidyl-serine confirming their anti-apoptotic capacity in those cells. Sub-G1 phase apparent in high glucose stressed cells was not seen in BC-HDD NPs treated cells. The present study reveals that BC-HDD NPs at ~20 fold lower concentration are as effective as BBR in preventing high glucose induced oxidative stress, mitochondrial depolarization and downstream events of apoptotic cell death. © 2014 Kapoor et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161429
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0089124
Rights: Attribution 4.0 International
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