Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0097555
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dc.titleEvaluation of a prednisolone acetate-loaded subconjunctival implant for the treatment of recurrent uveitis in a rabbit model
dc.contributor.authorAng M.
dc.contributor.authorNg X.
dc.contributor.authorWong C.
dc.contributor.authorYan P.
dc.contributor.authorChee S.-P.
dc.contributor.authorVenkatraman S.S.
dc.contributor.authorWong T.T.
dc.date.accessioned2019-11-05T00:37:19Z
dc.date.available2019-11-05T00:37:19Z
dc.date.issued2014
dc.identifier.citationAng M., Ng X., Wong C., Yan P., Chee S.-P., Venkatraman S.S., Wong T.T. (2014). Evaluation of a prednisolone acetate-loaded subconjunctival implant for the treatment of recurrent uveitis in a rabbit model. PLoS ONE 9 (5) : e97555. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0097555
dc.identifier.issn1932-6203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161411
dc.description.abstractAim: To assess the efficacy of a biodegradable, prednisolone acetate implant in a rabbit uveitis model. Methods: Randomized, controlled study of biodegradable microfilms preloaded with prednisolone acetate (PA) in a rabbit uveitis model. Experimental uveitis was induced by unilateral intravitreal injection of Mycobacterium tuberculosis H37Ra antigen (50 ug; 1 ug/uL) in preimmunized rabbits. PA-loaded poly[d,l-lactide-co-?-caprolactone] (PLC) microfilms (n = 10) and blank microfilms (n = 6) were implanted subconjunctivally. An estimate of PA release in vivo was calculated from measured residual PA amounts in microfilms after the rabbits were sacrificed. The eyes were clinically monitored for ocular inflammation for 28 days. Histopathological examination of the enucleated eyes was performed at the end of the study period. Results: In vitro studies revealed that sandwich PA-loaded microfilm formulations exhibited higher release kinetic compared to homogenous PA-loaded microfilms. The 60-40-60% microfilm released an average of 0.034 mg/day of PA over the period of 60 days in vitro; and we found that approximately 0.12 mg/day PA was released in vivo. Animals implanted with the PA-loaded microfilms exhibited significantly lowered median inflammatory scores when compared against the control group in this model for recurrent uveitis (P<0.001). The implants were clinically well tolerated by all the animals. Histology results showed no significant scarring or inflammation around the PA-loaded microfilms. Conclusion: Our pilot study demonstrated that a subconjunctival PA-loaded implant is effective in suppressing inflammation in the rabbit model of uveitis, by providing therapeutic levels of PA that attenuated the inflammatory response even after a rechallenge. Longer term studies are now needed to establish the therapeutic potential of such a delivery system for treatment of ocular inflammation. © 2014 Ang et al.
dc.sourceUnpaywall
dc.subjectpolycaprolactone
dc.subjectprednisolone acetate
dc.subjectprednisolone
dc.subjectprednisolone acetate
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbiodegradable implant
dc.subjectcontrolled study
dc.subjectdrug efficacy
dc.subjectdrug formulation
dc.subjectdrug implant
dc.subjectdrug release
dc.subjectdrug tolerability
dc.subjectenucleation
dc.subjectexperimental uveitis
dc.subjecteye inflammation
dc.subjecthistopathology
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectmale
dc.subjectmicrofilm
dc.subjectnonhuman
dc.subjectrabbit
dc.subjectrandomized controlled trial
dc.subjectrecurrent disease
dc.subjectscoring system
dc.subjectanalogs and derivatives
dc.subjectanimal
dc.subjectchemistry
dc.subjectconjunctiva
dc.subjectdrug administration route
dc.subjectinflammation
dc.subjectprostheses and orthoses
dc.subjectsurgery
dc.subjectuveitis
dc.subjectAnimals
dc.subjectConjunctiva
dc.subjectDrug Administration Routes
dc.subjectInflammation
dc.subjectMale
dc.subjectPrednisolone
dc.subjectProstheses and Implants
dc.subjectRabbits
dc.subjectUveitis
dc.typeArticle
dc.contributor.departmentDEAN'S OFFICE (DUKE-NUS MEDICAL SCHOOL)
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1371/journal.pone.0097555
dc.description.sourcetitlePLoS ONE
dc.description.volume9
dc.description.issue5
dc.description.pagee97555
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