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https://doi.org/10.1371/journal.pone.0067896
Title: | Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation | Authors: | Chen E. Tang M.K. Yao Y. Yau W.W.Y. Lo L.M. Yang X. Chui Y.L. Chan J. Lee K.K.H. |
Keywords: | actin binding protein adaptor protein bone morphogenetic protein fibroblast growth factor protein BRE small interfering RNA transforming growth factor beta unclassified drug animal cell article bone development BRE gene cell culture cell differentiation cell renewal chondrogenesis controlled study down regulation embryonic stem cell epigenetics Fgf5 gene FOXO1a gene gene gene expression gene silencing human human cell human tissue human umbilical cord perivascular progenitor cell microarray analysis multipotent stem cell nonhuman Oct4 gene pluripotent stem cell protein expression protein protein interaction proteomics signal transduction stem cell transcription regulation Animals Bone Morphogenetic Proteins Cell Differentiation Chemokines Chondrocytes Chondrogenesis Cytoskeletal Proteins Epigenesis, Genetic Fibroblast Growth Factors Gene Silencing Homeodomain Proteins Humans Mice Multipotent Stem Cells Nerve Tissue Proteins Octamer Transcription Factor-3 Oligonucleotide Array Sequence Analysis Osteoblasts Osteogenesis Proteome Proteomics RNA, Small Interfering Signal Transduction Stem Cells Transforming Growth Factor beta Umbilical Cord |
Issue Date: | 2013 | Citation: | Chen E., Tang M.K., Yao Y., Yau W.W.Y., Lo L.M., Yang X., Chui Y.L., Chan J., Lee K.K.H. (2013). Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation. PLoS ONE 8 (7) : e67896. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0067896 | Rights: | Attribution 4.0 International | Abstract: | BRE is a multifunctional adapter protein involved in DNA repair, cell survival and stress response. To date, most studies of this protein have been focused in the tumor model. The role of BRE in stem cell biology has never been investigated. Therefore, we have used HUCPV progenitor cells to elucidate the function of BRE. HUCPV cells are multipotent fetal progenitor cells which possess the ability to differentiate into a multitude of mesenchymal cell lineages when chemically induced and can be more easily amplified in culture. In this study, we have established that BRE expression was normally expressed in HUCPV cells but become down-regulated when the cells were induced to differentiate. In addition, silencing BRE expression, using BRE-siRNAs, in HUCPV cells could accelerate induced chondrogenic and osteogenic differentiation. Hence, we postulated that BRE played an important role in maintaining the stemness of HUCPV cells. We used microarray analysis to examine the transcriptome of BRE-silenced cells. BRE-silencing negatively regulated OCT4, FGF5 and FOXO1A. BRE-silencing also altered the expression of epigenetic genes and components of the TGF-?/BMP and FGF signaling pathways which are crucially involved in maintaining stem cell self-renewal. Comparative proteomic profiling also revealed that BRE-silencing resulted in decreased expressions of actin-binding proteins. In sum, we propose that BRE acts like an adaptor protein that promotes stemness and at the same time inhibits the differentiation of HUCPV cells. © 2013 Chen et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161286 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0067896 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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