Please use this identifier to cite or link to this item:
Title: Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation
Authors: Chen E.
Tang M.K.
Yao Y.
Yau W.W.Y. 
Lo L.M.
Yang X.
Chui Y.L.
Chan J.
Lee K.K.H.
Keywords: actin binding protein
adaptor protein
bone morphogenetic protein
fibroblast growth factor
protein BRE
small interfering RNA
transforming growth factor beta
unclassified drug
animal cell
bone development
BRE gene
cell culture
cell differentiation
cell renewal
controlled study
down regulation
embryonic stem cell
Fgf5 gene
FOXO1a gene
gene expression
gene silencing
human cell
human tissue
human umbilical cord perivascular progenitor cell
microarray analysis
multipotent stem cell
Oct4 gene
pluripotent stem cell
protein expression
protein protein interaction
signal transduction
stem cell
transcription regulation
Bone Morphogenetic Proteins
Cell Differentiation
Cytoskeletal Proteins
Epigenesis, Genetic
Fibroblast Growth Factors
Gene Silencing
Homeodomain Proteins
Multipotent Stem Cells
Nerve Tissue Proteins
Octamer Transcription Factor-3
Oligonucleotide Array Sequence Analysis
RNA, Small Interfering
Signal Transduction
Stem Cells
Transforming Growth Factor beta
Umbilical Cord
Issue Date: 2013
Citation: Chen E., Tang M.K., Yao Y., Yau W.W.Y., Lo L.M., Yang X., Chui Y.L., Chan J., Lee K.K.H. (2013). Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation. PLoS ONE 8 (7) : e67896. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: BRE is a multifunctional adapter protein involved in DNA repair, cell survival and stress response. To date, most studies of this protein have been focused in the tumor model. The role of BRE in stem cell biology has never been investigated. Therefore, we have used HUCPV progenitor cells to elucidate the function of BRE. HUCPV cells are multipotent fetal progenitor cells which possess the ability to differentiate into a multitude of mesenchymal cell lineages when chemically induced and can be more easily amplified in culture. In this study, we have established that BRE expression was normally expressed in HUCPV cells but become down-regulated when the cells were induced to differentiate. In addition, silencing BRE expression, using BRE-siRNAs, in HUCPV cells could accelerate induced chondrogenic and osteogenic differentiation. Hence, we postulated that BRE played an important role in maintaining the stemness of HUCPV cells. We used microarray analysis to examine the transcriptome of BRE-silenced cells. BRE-silencing negatively regulated OCT4, FGF5 and FOXO1A. BRE-silencing also altered the expression of epigenetic genes and components of the TGF-?/BMP and FGF signaling pathways which are crucially involved in maintaining stem cell self-renewal. Comparative proteomic profiling also revealed that BRE-silencing resulted in decreased expressions of actin-binding proteins. In sum, we propose that BRE acts like an adaptor protein that promotes stemness and at the same time inhibits the differentiation of HUCPV cells. © 2013 Chen et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0067896
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0067896.pdf5.48 MBAdobe PDF



Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons