Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0201826
DC Field | Value | |
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dc.title | ATP competes with PIP 2 for binding to gelsolin | |
dc.contributor.author | Szatmári D. | |
dc.contributor.author | Xue B. | |
dc.contributor.author | Kannan B. | |
dc.contributor.author | Burtnick L.D. | |
dc.contributor.author | Bugyi B. | |
dc.contributor.author | Nyitrai M. | |
dc.contributor.author | Robinson R.C. | |
dc.date.accessioned | 2019-11-01T08:13:16Z | |
dc.date.available | 2019-11-01T08:13:16Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Szatmári D., Xue B., Kannan B., Burtnick L.D., Bugyi B., Nyitrai M., Robinson R.C. (2018). ATP competes with PIP 2 for binding to gelsolin. PLoS ONE 13 (8) : e0201826. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0201826 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161218 | |
dc.description.abstract | Gelsolin is a severing and capping protein that targets filamentous actin and regulates filament lengths near plasma membranes, contributing to cell movement and plasma membrane morphology. Gelsolin binds to the plasma membrane via phosphatidylinositol 4,5-bisphosphate (PIP 2 ) in a state that cannot cap F-actin, and gelsolin-capped actin filaments are uncapped by PIP 2 leading to filament elongation. The process by which gelsolin is removed from PIP 2 at the plasma membrane is currently unknown. Gelsolin also binds ATP with unknown function. Here we characterize the role of ATP on PIP 2 -gelsolin complex dynamics. Fluorophore-labeled PIP 2 and ATP were used to study their interactions with gelsolin using steady-state fluorescence anisotropy, and Alexa488-labeled gelsolin was utilized to reconstitute the regulation of gelsolin binding to PIP 2 -containing phospholipid vesicles by ATP. Under physiological salt conditions ATP competes with PIP 2 for binding to gelsolin, while calcium causes the release of ATP from gelsolin. These data suggest a cycle for gelsolin activity. Firstly, calcium activates ATP-bound gelsolin allowing it to sever and cap F-actin. Secondly, PIP 2 -binding removes the gelsolin cap from F-actin at low calcium levels, leading to filament elongation. Finally, ATP competes with PIP 2 to release the calcium-free ATP-bound gelsolin, allowing it to undergo a further round of severing. © 2018 Szatmári et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | adenosine triphosphate | |
dc.subject | calcium | |
dc.subject | gelsolin | |
dc.subject | phosphatidylinositol 4,5 bisphosphate | |
dc.subject | phospholipid | |
dc.subject | sodium chloride | |
dc.subject | actin | |
dc.subject | adenosine triphosphate | |
dc.subject | cation | |
dc.subject | gelsolin | |
dc.subject | magnesium | |
dc.subject | phosphatidylinositol 4,5 bisphosphate | |
dc.subject | protein binding | |
dc.subject | actin filament | |
dc.subject | anisotropy | |
dc.subject | Article | |
dc.subject | cell membrane | |
dc.subject | controlled study | |
dc.subject | cytoplasm | |
dc.subject | enzyme activity | |
dc.subject | enzyme regulation | |
dc.subject | fluorescence | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | in vitro study | |
dc.subject | protein binding | |
dc.subject | protein function | |
dc.subject | protein protein interaction | |
dc.subject | protein secretion | |
dc.subject | steady state | |
dc.subject | animal | |
dc.subject | binding competition | |
dc.subject | Escherichia coli | |
dc.subject | kinetics | |
dc.subject | Leporidae | |
dc.subject | metabolism | |
dc.subject | polymerization | |
dc.subject | Actins | |
dc.subject | Adenosine Triphosphate | |
dc.subject | Animals | |
dc.subject | Binding, Competitive | |
dc.subject | Calcium | |
dc.subject | Cations | |
dc.subject | Cell Membrane | |
dc.subject | Escherichia coli | |
dc.subject | Gelsolin | |
dc.subject | Humans | |
dc.subject | Kinetics | |
dc.subject | Magnesium | |
dc.subject | Phosphatidylinositol 4,5-Diphosphate | |
dc.subject | Polymerization | |
dc.subject | Protein Binding | |
dc.subject | Rabbits | |
dc.type | Article | |
dc.contributor.department | DEPT OF BIOCHEMISTRY | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1371/journal.pone.0201826 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 13 | |
dc.description.issue | 8 | |
dc.description.page | e0201826 | |
Appears in Collections: | Staff Publications Elements |
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