Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0176345
Title: Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development
Authors: Papathanasiou P.
Petvises S.
Hey Y.-Y. 
Perkins A.C.
O'Neill H.C.
Keywords: animal cell
animal experiment
animal model
Article
bone marrow biopsy
cell maturation
embryo development
flow cytometry
gene mutation
hematopoietic stem cell
mouse
mutagenesis
myelopoiesis
nonhuman
phenotype
prevalence
animal
B lymphocyte
bone marrow cell
C57BL mouse
cell culture
coculture
cytology
dendritic cell
female
genetics
metabolism
point mutation
spleen
stroma cell
CD8 antigen
protein c Myb
Animals
Antigens, CD8
B-Lymphocytes
Bone Marrow Cells
Cells, Cultured
Coculture Techniques
Dendritic Cells
Female
Flow Cytometry
Hematopoietic Stem Cells
Mice
Mice, Inbred C57BL
Myeloid Cells
Myelopoiesis
Point Mutation
Proto-Oncogene Proteins c-myb
Spleen
Stromal Cells
Issue Date: 2017
Citation: Papathanasiou P., Petvises S., Hey Y.-Y., Perkins A.C., O'Neill H.C. (2017). Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development. PLoS ONE 12 (4) : e0176345. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0176345
Abstract: Booreana mice carrying the c-Myb308G point mutation were analyzed to determine changes in early hematopoiesis in the bone marrow and among mature cells in the periphery. This point mutation led to increased numbers of early hematopoietic stem and progenitor cells (HSPCs), with a subsequent reduction in the development of B cells, erythroid cells, and neutrophils, and increased numbers of myeloid cells and granulocytes. Myelopoiesis was further investigated by way of particular subsets affected. A specific question addressed whether booreana mice contained increased numbers of dendritic-like cells (L-DC subset) recently identified in the spleen, since L-DCs arise in vitro by direct differentiation from HSPCs co-cultured over splenic stroma. The non-lethal c-Myb mutation in booreana mice was associated with significantly lower representation of splenic CD8- conventional dendritic cells (cDCs), inflammatory monocytes, and neutrophils compared to wild-type mice. This result confirmed the bone marrow origin of progenitors for these subsets since c-Myb is essential for their development. Production of L-DCs and resident monocytes was not affected by the c-MybE308G mutation. These subsets may derive from different progenitors than those in bone marrow, and are potentially established in the spleen during embryogenesis. An alternative explanation may be needed for why there was no change in CD8+ cDCs in booreana spleen since these cells are known to derive from common dendritic progenitors in bone marrow. © 2017 Papathanasiou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161197
ISSN: 19326203
DOI: 10.1371/journal.pone.0176345
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