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Title: Immunomodulatory properties of polysaccharide-protein complex from lycium barbarum L.
Keywords: Lycium barbarum polysaccharide-protein complex; T cell; Macrophage; Dendritic cell
Issue Date: 15-Oct-2008
Citation: CHEN ZHISONG (2008-10-15). Immunomodulatory properties of polysaccharide-protein complex from lycium barbarum L.. ScholarBank@NUS Repository.
Abstract: Lycium barbarum L. (wolfberry) is a Chinese herbal medicine with various biological activities, such as hemopoeisis promotion, liver protection, and immunity improvement. This present study investigated the immunomodulatory properties of L. barbarum polysaccharide-protein complex (LBP) on T cells, macrophages, and dendritic cells (DCs). LBP was isolated from Lycium fruit and purified to five fractions, designated as LBPF1, LBPF2, LBPF3, LBPF4, and LBPF5. LBP is able to activate T cells. The active fractions are LBPF4 and LBPF5. LBP, LBPF4, and LBPF5 could significantly stimulate mouse T cell proliferation, prompt CD25 expression, activate transcription factors NFAT and AP-1, and induce IL-2 and IFN-N3 production. LBP also activates macrophages. LBP up-regulated the expression of CD40, CD80, CD86, and MHC class II molecules on peritoneal macrophages. LBP and LBPF1-5 triggered NF-N:B and AP-1 signaling, induced TNF-N1 production, and improved macrophage capacities in endocytosis and phagocytosis. LBP also induces phenotypic and functional maturation of DCs with strong immunogenicity. LBP upregulated the expression of CD40, CD80, CD86, and MHC class II molecules by mouse bone marrow-derived DCs (BMDCs) and splenic DCs, downregulated DC uptake of antigen, and enhanced DC allostimulatory activity and the production of IL-12p40 and p70. All its five fractions were active. LBP induced Th1 response in vivo. LBP-treated BMDCs enhanced Th1 and Th2 responses both in vitro and in vivo. In conclusion, the results showed that LBP is capable of activating macrophages, DCs, and T cells, indicating it can enhance both innate and adaptive immunity.
Appears in Collections:Ph.D Theses (Open)

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