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|Title:||A NOVEL BIODEGRADABLE TISSUE SCAFFOLD BASED ON CORE-SHELL STRUCTURED NANOFIBERS: INCORPORATING CONTROLLED DELIVERY SYSTEM||Authors:||FENG YU||Keywords:||core-shell structured nanofibers, PCL, PGA, BSA,VEGF, controlled delivery||Issue Date:||25-Feb-2007||Citation:||FENG YU (2007-02-25). A NOVEL BIODEGRADABLE TISSUE SCAFFOLD BASED ON CORE-SHELL STRUCTURED NANOFIBERS: INCORPORATING CONTROLLED DELIVERY SYSTEM. ScholarBank@NUS Repository.||Abstract:||In this study, core-shell structured nanofibers encapsulating model protein BSA and the growth factor VEGF were successfully fabricated by coaxial electrospinning. Two synthetic biodegradable PCL and PGA of different biodegradabilities were selected as the shell material for fabrication of BSA/PCL and BSA/PGA core-shell fibers, respectively. Graded flow rates were set for BSA when performing electrospinning, to obtain fibers encapsulating different amount of core component.The obtained core-shell nanofibers were evaluated as cell scaffolds with a function of biological agent delivery. The core-shell structure of the nanofibers was confirmed by TEM and ATR-FTIR spectroscopy. When cultured with human dermal fibroblasts (HDFs), results from SEM and fluorescence-staining examination indicated favorable interactions between the core-shell nanofibers and cells. Release kinetic studies of both BSA/PCL and BSA/PGA core-shell fibers in the absence of HDFs showed a gradual release of BSA without burst release for more than thirty and ten days, respectively. However, faster release of BSA was observed when HDFs were cultured on both fibers. To investigate the biological activity protection of the core-shell fibrous scaffold, the growth factor VEGF, which specifically stimulates proliferation of human coronary artery endothelial cells (HCAECs), was encapsulated into PGA nanofibers. Proliferation studies of HCAECs indicated that the VEGF released from the VEGF/PGA core-shell fibers retained its bioactivity of proliferation stimulation of HCAECs for at least 6 days. These results suggest that the core-shell structured nanofibers could possibly have good potentials as a novel controlled delivery system, as well as a tissue engineering scaffold encapsulating growth factors, enzymes, bioactive molecules, drugs, and even antibiotics for tissue repair and regeneration.||URI:||http://scholarbank.nus.edu.sg/handle/10635/15703|
|Appears in Collections:||Master's Theses (Open)|
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