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Title: Studies on translational mechanisms of RNA viruses
Keywords: translation initiation, RNA virus, frame-shifting, HCRSV, SARS-CoV
Issue Date: 8-Jan-2007
Citation: WANG XIAOXING (2007-01-08). Studies on translational mechanisms of RNA viruses. ScholarBank@NUS Repository.
Abstract: RNA viruses rely on host to translate their gene products. To fully utilize and expand their coding capacity, viruses have developed various mechanisms to regulate translation at different stages of translation, including initiation, elongation and termination. Programmed ribosomal frameshifting is one of such regulatory events during translation elongation. In this study, +1 and -1 frameshifting events induced by poly-uridines in SARS-CoV ORF 3a variants were identified and the mechanisms were explored. Results demonstrate that hepta- and octo-uridnes are highly efficient in inducing frameshifting independent of other stimulatory elements. Viruses also regulate their gene expression at the initiation stage. In this work, the coat protein p38 of hibiscus chlorotic ringspot virus (HCRSV) was shown to be regulated at translational level by multiple mechanisms including leaky scanning and IRES-mediated translation. Furthermore, ORF p27 overlapping with p38 was found to be initiated from a non-conventional start codon CUG. We also demonstrated that a small upstream ORF p9 could influence the expression of downstream ORFs. These data indicate that viral translation regulation is an intricate event.
Appears in Collections:Ph.D Theses (Open)

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