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Title: Baculovirus-mediated gene delivery for glioma therapy
Authors: LI FENG
Keywords: Baculovirus; Gene delivery; Glioma therapy; RNA interference;Gene expression;Bioluminescence
Issue Date: 14-Dec-2006
Citation: LI FENG (2006-12-14). Baculovirus-mediated gene delivery for glioma therapy. ScholarBank@NUS Repository.
Abstract: Gene therapy is a promising therapeutic strategy for gliomas, which are incurable by conventional approaches. In the current study, we investigated the feasibility of using baculovirus as a gene delivery vector for glioma therapy. A glial-specific promoter was created, which showed a high level expression of reporter genes in glioma cells in the context of baculovirus. The transgene expression level was further improved by flanking the expression cassette with inverted terminal repeats from adeno-associated virus. When therapeutic gene encoding diphtheria toxin A-chain was used, the inhibition of glioma cell growth was demonstrated in cell lines and in a rat C6 glioma xenograft model. RNA interference mediated by a recombinant baculoviral vector with a hybrid promoter (CMV enhancer/H1 promoter) was also studied and an effective knockdown of target gene expression was observed. These results show thatbaculoviral vectors might provide a new effective option for cancer gene therapy.
Appears in Collections:Master's Theses (Open)

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