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|Title:||Sterol-rich membrane domains and membrane-associated proteins in the fission yeast Schizosaccharomyces pombe||Authors:||VOLKER WACHTLER||Keywords:||plasma membrane, cytokinesis, Schizosaccharomyces pombe, actomyosin ring, Cdc15p, cell cycle||Issue Date:||27-Oct-2006||Citation:||VOLKER WACHTLER (2006-10-27). Sterol-rich membrane domains and membrane-associated proteins in the fission yeast Schizosaccharomyces pombe. ScholarBank@NUS Repository.||Abstract:||In this study, evidence is presented for the existence of sterol-rich domains in the plasma membrane of fission yeast cells. They localise in a cell cycle-dependent manner and play an important role in formation and maintenance of the actomyosin ring.Thereafter, it is shown that the FCH-domain protein Cdc15p has a pool of about 40% of its total cellular amount associated with membranes, and it is predominantly this membrane bound pool that becomes hypophosphorylated during cytokinesis.Finally, detailed investigation of the role of Cdc15p has revealed that Cdc15p contributes to actomyosin ring assembly at early and late stages of division. It is however only essential for ring formation at late stages since there exists an alternative, Mid1p-dependent pathway for ring assembly that is active at early stages. Hypophosphorylation of Cdc15p during cell division occurs in at least two distinct steps and is partially mediated through the phosphatase Clp1p/Flp1p.||URI:||http://scholarbank.nus.edu.sg/handle/10635/15555|
|Appears in Collections:||Ph.D Theses (Open)|
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