Please use this identifier to cite or link to this item: https://doi.org/10.1002/stem.2967
Title: Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids-Epigenetic Circuit.
Authors: Han, Zhenbo
Yu, Ying
Xu, Juan 
Bao, Zhengyi
Xu, Zihang
Hu, Jiancheng 
Yu, Meixi
Bamba, Djibril
Ma, Wenya
Ding, Fengzhi
Zhang, Lai
Jin, Mengyu
Yan, Gege
Huang, Qi
Wang, Xiuxiu
Hua, Bingjie
Yang, Fan 
Li, Yuan
Lei, Lei
Cao, Nan
Pan, Zhenwei
Cai, Benzhi
Keywords: Differentiation
H3K27me3
Iron homeostasis
NANOG
Pluripotent stem cells
Issue Date: Apr-2019
Publisher: Wiley
Citation: Han, Zhenbo, Yu, Ying, Xu, Juan, Bao, Zhengyi, Xu, Zihang, Hu, Jiancheng, Yu, Meixi, Bamba, Djibril, Ma, Wenya, Ding, Fengzhi, Zhang, Lai, Jin, Mengyu, Yan, Gege, Huang, Qi, Wang, Xiuxiu, Hua, Bingjie, Yang, Fan, Li, Yuan, Lei, Lei, Cao, Nan, Pan, Zhenwei, Cai, Benzhi (2019-04). Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids-Epigenetic Circuit.. Stem Cells 37 (4) : 489-503. ScholarBank@NUS Repository. https://doi.org/10.1002/stem.2967
Abstract: Iron homeostasis is crucial for a variety of biological processes, but the biological role of iron homeostasis in pluripotent stem cells (PSCs) remains largely unknown. The present study aimed to determine whether iron homeostasis is involved in maintaining the pluripotency of human PSCs (hPSCs). We found that the intracellular depletion of iron leads to a rapid downregulation of NANOG and a dramatic decrease in the self-renewal of hPSCs as well as spontaneous and nonspecific differentiation. Moreover, long-term depletion of iron can result in the remarkable cell death of hPSCs via apoptosis and necrosis pathways. Additionally, we found that the depletion of iron increased the activity of lipoprotein-associated phospholipase A2 (LP-PLA2) and the production of lysophosphatidylcholine, thereby suppressing NANOG expression by enhancer of zeste homolog 2-mediated trimethylation of histone H3 lysine 27. Consistently, LP-PLA2 inhibition abrogated iron depletion-induced loss of pluripotency and differentiation. Altogether, the findings of our study demonstrates that iron homeostasis, acting through glycerophospholipid metabolic pathway, is essential for the pluripotency and survival of hPSCs. Stem Cells 2019;37:489-503.
Source Title: Stem Cells
URI: https://scholarbank.nus.edu.sg/handle/10635/155483
ISSN: 10665099
15494918
DOI: 10.1002/stem.2967
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