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|Title:||Quality control of herbal medicine: chromatographic fingerprinting and screening for adulterants||Authors:||ZOU PENG||Keywords:||herbal medicine; quality control; fingerprinting; Isatis indigotica; adulteration; PDE-5 inhibitors||Issue Date:||10-Jul-2006||Citation:||ZOU PENG (2006-07-10). Quality control of herbal medicine: chromatographic fingerprinting and screening for adulterants. ScholarBank@NUS Repository.||Abstract:||Herbal medicine is widely used. In this thesis, two approaches to control the quality of herbal medicine and their products are studied, namely, chromatographic fingerprinting and screening for adulterants.HPLC and GC-MS methods were developed to obtain chromatographic fingerprints of roots of Isatis indigotica (also known as a??Ban-Lan-Gena??). The developed methods allow simple identification and comparisons of the I. indigotica roots from different sources. A rapid, selective, sensitive and novel LC-MS/MS method was developed to determine four active indoxyl derivatives (indican, isatin, indigotin and indirubin) in I. indigotica.LC-DAD and LC-MS/MS methods for the simultaneous detection and determination of three commercially available PDE-5 inhibitors (sildenafil, vardenafil, tadalafil) and their analogues (homosildenafil, hydroxyhomosildenafil and acetildenafil) were developed. The methods were successfully applied to one herbal product and six pre-mixed bulk powders for dietary supplements. All except tadalafil were detected in these seven samples. A tadalafil analogue was also isolated for the first time in a herbal product.In conclusion, the methods developed and successfully applied to herbal medicine and their products are found to be useful measures for the quality control of herbal medicine.||URI:||http://scholarbank.nus.edu.sg/handle/10635/15464|
|Appears in Collections:||Master's Theses (Open)|
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