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https://scholarbank.nus.edu.sg/handle/10635/153438
Title: | INVESTIGATING THE ROLE OF LONG NON-CODING RNAS IN INFLAMMATION | Authors: | BILAL UNAL | Keywords: | lncRNA, non-coding RNA, inflammation, NFkB, TNF, p38 pathway | Issue Date: | 9-Nov-2018 | Citation: | BILAL UNAL (2018-11-09). INVESTIGATING THE ROLE OF LONG NON-CODING RNAS IN INFLAMMATION. ScholarBank@NUS Repository. | Abstract: | Many proteins that regulate signaling downstream of master transcription factor NFkB have been identified. However, ~1% of the human genome encodes proteins, with a majority coding for various classes of non-coding RNAs. Long non-coding RNAs (lncRNAs) constitute a significant proportion of non-coding RNAs, and their function in regulation of NFkB biology is largely unexplored. Here, RNA-sequencing was used to identify lncRNAs regulated by NFkB pathway. From this genetic-screen, an evolutionarily conserved mouse Gm16685 lncRNA was identified. CRISPR-Cas9 mediated targeting of Gm16685 and its human homolog loc105375914 lncRNAs mitigated activation of p38 and NFkB responses, which led to reduced TNF levels. Loss of Gm16685 reduced tumorigenesis and metastasis in vivo. Moreover, to identify protein interactor partners of this novel lncRNA, RNA pull-down followed by mass spectrometry was performed. The candidate protein partners from mass spectrometry can be further validated for future mechanistic studies and their involvements with these lncRNAs in inflammation. | URI: | https://scholarbank.nus.edu.sg/handle/10635/153438 |
Appears in Collections: | Master's Theses (Open) |
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