Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/15341
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dc.titleThe mechanisms of lactacystin-induced apoptosis of mouse primary cortical neurons
dc.contributor.authorCHOY MENG SHYAN
dc.date.accessioned2010-04-08T10:52:30Z
dc.date.available2010-04-08T10:52:30Z
dc.date.issued2006-08-09
dc.identifier.citationCHOY MENG SHYAN (2006-08-09). The mechanisms of lactacystin-induced apoptosis of mouse primary cortical neurons. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/15341
dc.description.abstractExposure of the proteasome inhibitor lactacystin to mouse primary cortical neurons induced apoptosis and caused the accumulation of tumor suppressor PTEN in the plasma membrane. However, microarray analysis of neurons exposed to lactacystin reveals the early up-regulation of genes in response to unfolded proteins and endoplasmic reticulum (ER) stress. A mechanism of apoptotic cell death through the abnormal accumulation of protein and ER stress is suggested. The lipid profile of the neural membrane reveals the accumulation of ceramides and N-acyl phosphatidylethanolamines (NAPEs) during lactacystin-induced apoptosis. The accumulation of NAPEs during apoptosis has not been reported previously. Epigallocatechin-3-gallate (EGCG), a naturally occurring proteasome inhibitor from green tea extract was able to induce apoptosis in cultured neurons at 25 A?M. At a lower concentration (1 A?M), EGCG was able to cause the up-regulation of genes encoding proteasome subunits and ubiquitin, suggesting a potential neuroprotective effect against neurodegeneration.
dc.language.isoen
dc.subjectProteasome inhibition, lactacystin, apoptosis, PTEN, microarray, lipid profile
dc.typeThesis
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.supervisorCHEUNG NAM SANG
dc.contributor.supervisorLEE YIU WAH
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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