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Title: Cell-based Profiling of Lipid-Protein Interactions
Keywords: Chemical biology
Lipid-protein interactions
Issue Date: 2-Apr-2018
Citation: PHUA ZHENG YEN (2018-04-02). Cell-based Profiling of Lipid-Protein Interactions. ScholarBank@NUS Repository.
Abstract: Lipids are essential building blocks of cellular membranes. They are also key in cellular processes, such as cell signaling and maintaining membrane integrity. Alteration in lipid homeostasis has been known to cause pathologies such as cancer. Therefore, it is important to understand lipid-mediated pathways in cells. One way to do this is through the study of lipid-protein interactions and several groups have employed lipid probes to photocrosslink their binding proteins for proteomics profiling. However, to date only fatty acids and no lipid head groups containing diazirine have been reported to study mainly transmembrane and not peripheral proteins. Therefore this project explores the chemoenzymatic incorporation of diazirine-containing lipid head groups where phosphatidylalcohols transphosphatidylated from phosphatidylcholine were validated with lipid thin-layer chromatography and mass spectrometry as well as imaged in live mammalian cells. At the same time, the chemical synthesis of a sialic acid probe that could be used to form bifunctional gangliosides via the metabolic double incorporation approach was attempted.
Appears in Collections:Bachelor's Theses

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