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Title: Engineering aggregates with chemical linkers for tissue engineering application
Authors: HE LIJUAN
Keywords: cell surface modification, chemical linker, cell aggregates, suspension culture, fluorescence, tissue engineering
Issue Date: 7-Aug-2006
Citation: HE LIJUAN (2006-08-07). Engineering aggregates with chemical linkers for tissue engineering application. ScholarBank@NUS Repository.
Abstract: Combining principles from tissue engineering, cell surface engineering and chemistry, this thesis explored a novel way to engineer artificial multi-cellular structures. Taking the advantage of the functions of reacting with non-native groups on cell surface and that of linking cells, we designed different kinds of chemicals that were shown to aggregate cells fast and efficiently. Further study on PEI-2000-hy disclosed the charge interaction between the linker and negatively charged cell surface as an important factor for designing linkers. Although the linkers were found to disappear from the cell surface by tracking the tagged fluorescence, the cell aggregates tend to grow more compact and showed higher viability and functionality in suspension culture. The principles underlying this phenomenon require further study. Finally, we proved that the shape and size of the multi-cellular structure could be controlled by micropatterning and micromanipulation, which further validated the usage of this engineering aggregates approach.
Appears in Collections:Master's Theses (Open)

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