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Title: From protein self-assembly to crystallization
Keywords: protein self-assembly, protein crystallization, nonionic surfactant, molecular interaction, interfacial kinetics, prediction criterion
Issue Date: 25-Apr-2006
Citation: JIA YANWEI (2006-04-25). From protein self-assembly to crystallization. ScholarBank@NUS Repository.
Abstract: Protein crystallization has attracted much attention due to its wide application in life science. However, it is difficult to crystallize protein and it is often a tedious task to identify optimal crystallization conditions. The purpose of this thesis is to develop a new understanding on the mechanism of protein crystallization based on protein interactions and to develop a prediction criterion based on the kinetics of protein self-assembly versus transport. The interfacial kinetics of protein crystallization shows that the competition between the protein volume transport and surface integration determines whether crystals or amorphous aggregates will occur. Based on this, a kinetic coefficient was identified, which allows a precise prediction of protein crystallization. Apart from this, the effect of nonionic surfactant on protein crystallization is also examined. Nonionic surfactant tetraoxyethylene glycol monooctyl ether (C8E4) could adsorb on the hydrophobic parts of the protein molecules and changes the molecular interactions, which permits a favorable tuning of crystallization conditions. This study would benefit investigations on drug design, drug delivery, as well as the determination of protein structure.
Appears in Collections:Ph.D Theses (Open)

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