Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.actbio.2019.02.001
Title: Thin peptide hydrogel membranes suitable as scaffolds for engineering layered biostructures
Authors: KARTHIKEYAN KANDASAMY 
Purnamawati, Kristy
Seow, Wei Yang
Sun, William
Hauser, Charlotte A.E.
Keywords: Disulfide bonds
Layered biostructures
Membranes
Scaffolds
Tissue engineering
Ultrashort peptide hydrogel
Issue Date: 2-Feb-2019
Publisher: ELSEVIER
Citation: KARTHIKEYAN KANDASAMY, Purnamawati, Kristy, Seow, Wei Yang, Sun, William, Hauser, Charlotte A.E. (2019-02-02). Thin peptide hydrogel membranes suitable as scaffolds for engineering layered biostructures. Acta Materialia forthcoming. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2019.02.001
Abstract: A short tetramer peptide, Ac-IVKC, spontaneously formed a hydrogel in water. Disulfide bonds were introduced via hydrogen peroxide (H2O2)-assisted oxidation, resulting in (Ac-IVKC)2 dimers. The extent of disulfide bond formation and gel stiffness increased with the amount of H2O2 used and 100% dimerization was achieved with 0.2% H2O2. The resultant gel achieved an elastic modulus of ∼0.9 MPa, which to our knowledge, has not been reported for peptide-based hydrogels. The enhanced mechanical property enabled the fabrication of thin and transparent membranes. The hydrogel could also be handled with forceps at mm thickness, greatly increasing its ease of physical manipulation. Excess H2O2 was removed and the membrane was then infused with cell culture media. Various cells, including primary human corneal stromal and epithelial cells, were seeded onto the hydrogel membrane and demonstrated to remain viable. Depending on the intended application, specific cell combination or membrane stacking order could be used to engineer layered biostructures. STATEMENT OF SIGNIFICANCE: A short tetramer peptide - Ac-IVKC - spontaneously formed a hydrogel in water and disulfide bonds were introduced via hydrogen peroxide (H2O2)-assisted oxidation. The extent of disulfide-bond formation and gel stiffness were modulated by the amount of H2O2. At maximum disulfide-bond formation, the hydrogel achieved an elastic modulus of ∼0.9 MPa, which to our knowledge, has not been reported for peptide-based hydrogels. The enhanced mechanical property enabled the fabrication of thin transparent membranes that can be physically manipulated at mm thickness. The gels also supported 3D cell growth, including primary human corneal stromal and epithelial cells. Depending on the intended application, specific combination of cells or individual membrane stacking order could be used to engineer layered biostructures.
Source Title: Acta Materialia
URI: http://scholarbank.nus.edu.sg/handle/10635/152191
ISSN: 17427061
DOI: 10.1016/j.actbio.2019.02.001
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