Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms19092553
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dc.titleDNA methylation profiling of breast cancer cell lines along the epithelial mesenchymal spectrum—Implications for the choice of circulating tumour DNA methylation markers
dc.contributor.authorLe A.V.-P.
dc.contributor.authorSzaumkessel M.
dc.contributor.authorTan T.Z.
dc.contributor.authorThiery J.-P.
dc.contributor.authorThompson E.W.
dc.contributor.authorDobrovic A.
dc.date.accessioned2019-03-11T06:42:08Z
dc.date.available2019-03-11T06:42:08Z
dc.date.issued2018
dc.identifier.citationLe A.V.-P., Szaumkessel M., Tan T.Z., Thiery J.-P., Thompson E.W., Dobrovic A. (2018). DNA methylation profiling of breast cancer cell lines along the epithelial mesenchymal spectrum—Implications for the choice of circulating tumour DNA methylation markers. International Journal of Molecular Sciences 19 (9) : 2553. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms19092553
dc.identifier.issn16616596
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/152182
dc.description.abstract(1) Background: Epithelial–mesenchymal plasticity (EMP) is a dynamic process whereby epithelial carcinoma cells reversibly acquire morphological and invasive characteristics typical of mesenchymal cells. Identifying the methylation differences between epithelial and mesenchymal states may assist in the identification of optimal DNA methylation biomarkers for the blood-based monitoring of cancer. (2) Methods: Methylation-sensitive high-resolution melting (MS-HRM) was used to examine the promoter methylation status of a panel of established and novel markers in a range of breast cancer cell lines spanning the epithelial–mesenchymal spectrum. Pyrosequencing was used to validate the MS-HRM results. (3) Results: VIM, DKK3, and CRABP1 were methylated in the majority of epithelial breast cancer cell lines, while methylation of GRHL2, MIR200C, and CDH1 was restricted to mesenchymal cell lines. Some markers that have been used to assess minimal residual disease such as AKR1B1 and APC methylation proved to be specific for epithelial breast cell lines. However, RASSF1A, RAR?, TWIST1, and SFRP2 methylation was seen in both epithelial and mesenchymal cell lines, supporting their suitability for a multimarker panel. (4) Conclusions: Profiling DNA methylation shows a distinction between epithelial and mesenchymal phenotypes. Understanding how DNA methylation varies between epithelial and mesenchymal phenotypes may lead to more rational selection of methylation-based biomarkers for circulating tumour DNA analysis. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
dc.publisherMDPI AG
dc.sourceScopus
dc.subjectBiomarkers
dc.subjectBreast cancer
dc.subjectCirculating tumour DNA
dc.subjectDNA methylation
dc.subjectEpithelial
dc.subjectMesenchymal plasticity
dc.subjectMethylation-sensitive high-resolution melting (MS-HRM)
dc.subjectMinimal residual disease
dc.subjectPyrosequencing
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.3390/ijms19092553
dc.description.sourcetitleInternational Journal of Molecular Sciences
dc.description.volume19
dc.description.issue9
dc.description.page2553
dc.published.statePublished
dc.grant.idCG-10-04
dc.grant.id1027527
dc.grant.idCG-08-07
dc.grant.idCG-12-07
dc.grant.fundingagencyNational Collaborative Research Program of the National Breast Cancer Foundation
dc.grant.fundingagencyNational Health and Medical Research Council of Australia
dc.grant.fundingagencyCancer Council of Victoria
dc.grant.fundingagencyVictorian Cancer Agency
dc.grant.fundingagencyUniversity of Melbourne
dc.grant.fundingagencyCancer Therapeutics CRC (CTx)
dc.grant.fundingagencyVictorian Government Operational and Infrastructure
dc.grant.fundingagencyAustralian Government
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