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Title: Molecular analysis of hibiscus chlorotic ringspot virus coat protein mediated suppression of gene silencing
Keywords: HCRSV, Coat Protein, PTGS, , siRNA, RDR6, microRNA,
Issue Date: 19-Apr-2006
Citation: MENG CHUNYING (2006-04-19). Molecular analysis of hibiscus chlorotic ringspot virus coat protein mediated suppression of gene silencing. ScholarBank@NUS Repository.
Abstract: Gene silencing and virus-encoded gene silencing suppressors are defense and counterdefense strategies evolved by host and pathogens. The coat protein (CP) of Hibiscus chlorotic ringspot virus (HCRSV) was identified to be a strong gene silencing suppressor which suppresses PTGS at the early initiation step. CP mutations resulted from serial passages of HCRSV in its local lesion host also showed a significantly reduced suppression function. HCRSV CP could not suppress dsRNA-induced PTGS. The suppression of CP on PTGS may involve an RdRp-related early initiation step. HCRSV CP-transgenic Arabidopsis showed phenotypes of reduced rosette leave number, early flowering and reduced fertility which resemble the phenotypes in RDR6, SGS3 and ZIP mutants. In CP-transgenic Arabidopsis, miR171 and miR172 accumulation were increased, miR173 not changed and trans-acting siRNA siR255 decreased, suggesting that HCRSV CP may suppress the ta-siRNAs maturation at the RDR6-SGS3 dependent conversion of single-stranded TAS RNAs into dsRNAs.
Appears in Collections:Ph.D Theses (Open)

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