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|Title:||Characterization of a type III secretion system and other virulence-associated genes in Aeromonas hydrophila||Authors:||YU HONGBING||Keywords:||type III secretion system, virulence factors, effector, multi-factorial, proteomics approach, cross-talk||Issue Date:||22-May-2006||Citation:||YU HONGBING (2006-05-22). Characterization of a type III secretion system and other virulence-associated genes in Aeromonas hydrophila. ScholarBank@NUS Repository.||Abstract:||A type III secretion system (TTSS) in A. hydrophila was identified for the first time. Inactivation of aopB or aopD within this TTSS led to decreased cytotoxicity in carp epithelial cells, increased phagocytosis and reduced virulence in blue gourami fish. Two effectors, AopE and AopH, were identified and shown to be secreted in a TTSS-dependent manner. These two effectors induce cytotoxicity in HeLa cells after transfection. We also investigated the multi-factorial pathogenicity of A. hydrophila by infecting blue gourami fish with various deletion mutants and showed that the TTSS is one of the key virulence factors in A. hydrophila pathogenesis. In addition, the extracellular virulence factors were characterized by a proteomics approach. Results suggested that a serine protease is involved in the processing of several secreted enzymes such as hemolysin, GCAT and metalloprotease. Interestingly, a cross-talk between the lateral flagellar secretion system and the TTSS was revealed in A. hydrophila.||URI:||http://scholarbank.nus.edu.sg/handle/10635/15185|
|Appears in Collections:||Ph.D Theses (Open)|
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