Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/151671
Title: NANOSCALE STRUCTURAL ORGANIZATION OF INTEGRIN-BASED ADHESION COMPLEXES AND ACTIN CYTOSKELETON IN MOUSE EMBRYONIC STEM CELLS
Authors: XIA SHUMIN
ORCID iD:   orcid.org/0000-0003-2792-5794
Keywords: Focal adhesions, Actin cortex, Embryonic stem cells, Super-resolution microscopy, Atomic force microscopy, Cell stiffness
Issue Date: 3-Aug-2018
Citation: XIA SHUMIN (2018-08-03). NANOSCALE STRUCTURAL ORGANIZATION OF INTEGRIN-BASED ADHESION COMPLEXES AND ACTIN CYTOSKELETON IN MOUSE EMBRYONIC STEM CELLS. ScholarBank@NUS Repository.
Abstract: Mechanical cues influence pluripotent stem cell differentiation but the underlying mechanisms are not well understood. Mouse embryonic stem cells (mESCs) exhibit unusual cytomechanical properties including low cell stiffness and attenuated responses to substrate rigidity, but the structural basis remains obscure. Here I investigated the integrin-based focal adhesions (FAs) and cortical actin cytoskeleton in mESCs using super-resolution microscopy. I observed that mESC FAs exhibited a multi-layer nanoscale architecture comparable to FAs of differentiated cells, but that the mESC cortex adopted a remarkably sparse architecture that largely exclude myosin II. Combining structural and mechanical measurements with molecular perturbation, our results suggested that mutual competition between formins, Arp2/3, and actin capping protein governed cortical structure and mechanics, in part through transient aster-like intermediate structures. This generated low network density that physically excluded myosin II from the cortex. The distinctive actin cytoskeletal organization in mESCs thus prescribes their unusual cell mechanical properties.
URI: http://scholarbank.nus.edu.sg/handle/10635/151671
Appears in Collections:Ph.D Theses (Open)

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