Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/15163
DC Field | Value | |
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dc.title | Protein complex studies on calmodulin - GAP-43 (neuromodulin) | |
dc.contributor.author | LIU BO | |
dc.date.accessioned | 2010-04-08T10:50:40Z | |
dc.date.available | 2010-04-08T10:50:40Z | |
dc.date.issued | 2005-12-28 | |
dc.identifier.citation | LIU BO (2005-12-28). Protein complex studies on calmodulin - GAP-43 (neuromodulin). ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/15163 | |
dc.description.abstract | Calmodulin (CaM) is a ubiquitous Ca2+-binding protein found in all eukaryotic cells. It interacts with a wide variety of proteins and modulates their functions. In low intracellular Ca2+ concentration, Ca2+-free CaM (apoCaM) interacts with proteins containing IQ motif. GAP-43 (Neuromodulin) is a neuron specific growth-associated protein containing IQ-motif as its CaM-binding domain. It is considered to be a major determinant of synaptic development and plasticity, and has been implicated in molecular mechanisms underlying learning and memory. PKC phosphorylation has important effects on the interaction between CaM and GAP-43.CaM and a GAP-43 truncate (GAP-43t) were overexpressed and purified. Protein complex of apoCaM and GAP-43t was obtained by GTA crosslinking and identified by SDS-PAGE analysis. This protein complex provides a new approach to the studies on both the CaM a?? GAP-43 interaction and the apoCaM a?? IQ motif interaction. Further studies on the protein complex will provide significant insights into both interactions. | |
dc.language.iso | en | |
dc.subject | protein complex, Calmodulin, GAP-43, IQ motif, GTA, crosslinking | |
dc.type | Thesis | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.supervisor | SHEU FWU-SHAN | |
dc.contributor.supervisor | JAYARAMAN SIVARAMAN | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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Thesis.pdf | 2.74 MB | Adobe PDF | OPEN | None | View/Download |
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