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Title: | CHARACTERIZATION OF THE INNER MEMBRANE ABC TRANSPORTER COMPLEX IMPORTANT FOR MAINTENANCE OF OUTER MEMBRANE LIPID ASYMMETRY IN ESCHERICHIA COLI | Authors: | THONG SHUHUA | Keywords: | ABC transporter, Outer Membrane, Gram-negative bacteria, Phospholipid transport, Lipid asymmetry, Protein complex assembly | Issue Date: | 2-Aug-2018 | Citation: | THONG SHUHUA (2018-08-02). CHARACTERIZATION OF THE INNER MEMBRANE ABC TRANSPORTER COMPLEX IMPORTANT FOR MAINTENANCE OF OUTER MEMBRANE LIPID ASYMMETRY IN ESCHERICHIA COLI. ScholarBank@NUS Repository. | Abstract: | The outer membrane (OM) of Gram-negative bacteria is an asymmetric lipid bilayer consisting of lipopolysaccharides (LPS) and phospholipids (PLs) in the outer and inner leaflets, respectively. It is essential for growth, and its unique lipid asymmetry renders it an effective permeability barrier against toxic compounds including antibiotics. In Escherichia coli, the OmpC-Mla system is important for maintenance of OM lipid asymmetry, and is proposed to actively prevent PL accumulation in the outer leaflet of the OM by extracting and transporting aberrantly localized PLs back to the inner membrane (IM). This process is supported by a putative ATP-binding cassette (ABC) transporter in the IM, whose composition and function are not known. In this thesis, we describe the biochemical characterization of this ABC transporter complex. Overall, our work provides mechanistic insights into how the MlaFEDB complex participates in ensuring active retrograde PL transport to maintain OM lipid asymmetry. | URI: | http://scholarbank.nus.edu.sg/handle/10635/151592 |
Appears in Collections: | Ph.D Theses (Open) |
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