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|Title:||THE ROLE OF HETEROCHROMATIN PROTEIN 1 BINDING PARTNER 3, HP1BP3, IN THE CARDIOMYOCTYE STRESS RESPONSE USING THE MURINE MODEL OF PRESSURE OVERLOAD||Authors:||CHAN XUELI CHERYL||Keywords:||Heterochromatin, Epigenetics, Heart Failure, Cardiomyocyte, Single Cell Transcriptomics, TAC model||Issue Date:||24-Aug-2018||Citation:||CHAN XUELI CHERYL (2018-08-24). THE ROLE OF HETEROCHROMATIN PROTEIN 1 BINDING PARTNER 3, HP1BP3, IN THE CARDIOMYOCTYE STRESS RESPONSE USING THE MURINE MODEL OF PRESSURE OVERLOAD. ScholarBank@NUS Repository.||Abstract:||An epigenetic protein Heterochromatin Protein 1 Binding Partner 3, HP1BP3, was uncovered through single nuclear sequencing of healthy and diseased CMs. It is centrally located within a network of disease-related cardiac genes, suggesting its role in disease gene regulation. It is up-regulated in a sub-population of diseased CMs, but its differential expression is masked in bulk cardiac tissue. Therefore, to investigate its role in regulating cardiomyocyte stress genes, a series of in vitro knockdown (KD) and over-expression experiments were first performed and then brought forth to in vivo using a unique cardiomyocyte-specific, cardiotrophic rAAV9-mediated KD technique. In vitro results showed changes in apoptosis and stress genes regulation while in vivo results showed maintenance of heart function through Hp1bp3-KD cardiomyocytes that were hypertrophic and hypercontractile. The thesis also covers an in-depth investigation of the mouse model of pressure overload that is ubiquitously used for studying cardiac hypertrophy and heart failure.||URI:||http://scholarbank.nus.edu.sg/handle/10635/151531|
|Appears in Collections:||Ph.D Theses (Open)|
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