Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/151531
Title: THE ROLE OF HETEROCHROMATIN PROTEIN 1 BINDING PARTNER 3, HP1BP3, IN THE CARDIOMYOCTYE STRESS RESPONSE USING THE MURINE MODEL OF PRESSURE OVERLOAD
Authors: CHAN XUELI CHERYL
Keywords: Heterochromatin, Epigenetics, Heart Failure, Cardiomyocyte, Single Cell Transcriptomics, TAC model
Issue Date: 24-Aug-2018
Citation: CHAN XUELI CHERYL (2018-08-24). THE ROLE OF HETEROCHROMATIN PROTEIN 1 BINDING PARTNER 3, HP1BP3, IN THE CARDIOMYOCTYE STRESS RESPONSE USING THE MURINE MODEL OF PRESSURE OVERLOAD. ScholarBank@NUS Repository.
Abstract: An epigenetic protein Heterochromatin Protein 1 Binding Partner 3, HP1BP3, was uncovered through single nuclear sequencing of healthy and diseased CMs. It is centrally located within a network of disease-related cardiac genes, suggesting its role in disease gene regulation. It is up-regulated in a sub-population of diseased CMs, but its differential expression is masked in bulk cardiac tissue. Therefore, to investigate its role in regulating cardiomyocyte stress genes, a series of in vitro knockdown (KD) and over-expression experiments were first performed and then brought forth to in vivo using a unique cardiomyocyte-specific, cardiotrophic rAAV9-mediated KD technique. In vitro results showed changes in apoptosis and stress genes regulation while in vivo results showed maintenance of heart function through Hp1bp3-KD cardiomyocytes that were hypertrophic and hypercontractile. The thesis also covers an in-depth investigation of the mouse model of pressure overload that is ubiquitously used for studying cardiac hypertrophy and heart failure.
URI: http://scholarbank.nus.edu.sg/handle/10635/151531
Appears in Collections:Ph.D Theses (Open)

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