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Title: Functional analysis of WNT3 in zebrafish neural development
Keywords: Wnt3, zebrafish, b-catenin signaling pathway and neurogenesis.
Issue Date: 9-Jan-2006
Citation: TEH CATHLEEN (2006-01-09). Functional analysis of WNT3 in zebrafish neural development. ScholarBank@NUS Repository.
Abstract: The homozygous nonsense mutation of WNT3 in human (Q83X) results in autosomal recessive tetra amelia (loss of four limbs) and affected fetuses exhibit craniofacial, nervous system, pulmonary, skeletal, and urogenital anomalies. This thesis aims to define the role of Wnt3 in neural development of zebrafish to shed light into malformation of the brain that exists but not characterized in human WNT3 mutants. We isolated zebrafish wnt3 and detailed its expression pattern during embryogenesis. Areas with strong expression lie mainly in dorsal brain and include the ventral epithalamus, dorsal thalamus, optic tectum, cerebellum and hindbrain. Our functional analysis involving site-specific overexpression of Wnt3 and panembryonic knockdown of its function by morpholino demonstrated that Wnt3 is required for formation of prosomere 2, optic tectum, cerebellum and branchial arches and further suggest that these developmental processes involved required the activation of Wnt3/b-catenin signaling pathway.
Appears in Collections:Ph.D Theses (Open)

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