Proteomic analysis of HCC-M and HEPG2 cells after treatment by butyrate

Abstract

The present study is focused on the response of the two cell lines, HCC-M and HepG2, to butyrate treatment by monitoring the alteration in protein expression by a proteomic approach. Both cell linesa?? viabilities were inhibited by butyrate in a dose- and time-dependent manner. 5mM butyrate could completely inhibit HCC-M cell growth, while partially to HepG2. HCC-M cells showed highest apoptotic changes under 5mM butyrate treatment for 48 hours, whereas for HepG2, the apoptotic percentage kept increasing but lower than the one of HCC-M until 72 hours. Protein expression differences between the control and treated cells of both hepatoma cell lines were analyzed by 2-dimensional electrophoresis followed by identification by mass spectrometry. Up-regulation of RGS19 interaction protein1 and down-regulation of calmodulin were found only in butyrate-treated HCC-M cell. These proteins may provide insight into the molecular basis of the higher sensitivity of HCC-M to butyrate treatment.