Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/150886
Title: CONTROL OF ANTIBIOTIC RESISTANCE BY UV AND CHLORINATION DISINFECTION PROCESSES
Authors: LIU XIAOCHEN
Keywords: Antibiotic resistance, disinfection, UV, chlorination, sul1 gene, horizontal gene transfer
Issue Date: 21-Aug-2018
Citation: LIU XIAOCHEN (2018-08-21). CONTROL OF ANTIBIOTIC RESISTANCE BY UV AND CHLORINATION DISINFECTION PROCESSES. ScholarBank@NUS Repository.
Abstract: This thesis evaluated the effect of UV and chlorination, two of the most frequently used disinfection methods, on the control of antibiotic resistance. Sulphonamide resistance sul1 genes are selected as targeted ARGs. Sequential UV-Cl2 process and simultaneous combination UV/Cl2 process showed higher effectiveness in deactivating sul1 genes compared with individual UV, free chlorine and other combined processes, and synergy values up to 0.489 and 0.609 log were observed for these combined processes. The mechanisms study shows the effect of DNA size was the major reason for the improved performance of UV-Cl2 process, while in UV/Cl2 process, both formation of ROS and the effect of DNA size contributed to the synergetic effect. Regarding the elimination of ARGs horizontal transfer, both UV and chlorination was able to reduce the transformation efficiency of the targeted sul1 genes. However, due to their different impacts on changing plasmid conformations, UV and free chlorine showed different trends in the elimination of ARGs transformation.
URI: http://scholarbank.nus.edu.sg/handle/10635/150886
Appears in Collections:Ph.D Theses (Open)

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