Please use this identifier to cite or link to this item:
Title: Natural variation of histone modification and its impact on gene expression in the rat genome
Authors: Rintisch C.
Heinig M.
Bauerfeind A.
Schafer S.
Mieth C.
Patone G.
Hummel O.
Chen W.
Cook S. 
Cuppen E.
Colom‚-Tatch‚ M.
Johannes F.
Jansen R.C.
Neil H.
Werner M.
Pravenec M.
Vingron M.
Hubner N.
Issue Date: 2014
Publisher: Cold Spring Harbor Laboratory Press
Citation: Rintisch C., Heinig M., Bauerfeind A., Schafer S., Mieth C., Patone G., Hummel O., Chen W., Cook S., Cuppen E., Colom‚-Tatch‚ M., Johannes F., Jansen R.C., Neil H., Werner M., Pravenec M., Vingron M., Hubner N. (2014). Natural variation of histone modification and its impact on gene expression in the rat genome. Genome Research 24 (6) : 942-953. ScholarBank@NUS Repository.
Abstract: Histone modifications are epigenetic marks that play fundamental roles in many biological processes including the control of chromatin-mediated regulation of gene expression. Little is known about interindividual variability of histone modification levels across the genome and to what extent they are influenced by genetic variation. We annotated the rat genome with histone modification maps, identified differences in histone trimethyl-lysine levels among strains, and described their underlying genetic basis at the genome-wide scale using ChIP-seq in heart and liver tissues in a panel of rat recombinant inbred and their progenitor strains. We identified extensive variation of histone methylation levels among individuals and mapped hundreds of underlying cis- and trans-acting loci throughout the genome that regulate histone methylation levels in an allele-specific manner. Interestingly, most histone methylation level variation was trans-linked and the most prominent QTL identified influenced H3K4me3 levels at 899 putative promoters throughout the genome in the heart. Cis- acting variation was enriched in binding sites of distinct transcription factors in heart and liver. The integrated analysis of DNA variation together with histone methylation and gene expression levels showed that histoneQTLs are an important predictor of gene expression and that a joint analysis significantly enhanced the prediction of gene expression traits (eQTLs). Our data suggest that genetic variation has a widespread impact on histone trimethylation marks that may help to uncover novel genotype-phenotype relationships. ? 2014 Rintisch et al.
Source Title: Genome Research
ISSN: 10889051
DOI: 10.1101/gr.169029.113
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Genome Res.-2014-Rintisch-gr.169029.113.pdf1.6 MBAdobe PDF




checked on May 18, 2019

Page view(s)

checked on May 23, 2019


checked on May 23, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.