Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/150683
Title: EXPLOITING THE PROTEIN CORONA AROUND GOLD NANOPARTICLES IN MODULATION OF PROTEIN EXPRESSION
Authors: CHAN KIAN PING
ORCID iD:   orcid.org/0000-0003-2202-9608
Keywords: Gold nanoparticles, protein corona, hybridization, p53 peptide, mRNA translation
Issue Date: 20-Aug-2018
Citation: CHAN KIAN PING (2018-08-20). EXPLOITING THE PROTEIN CORONA AROUND GOLD NANOPARTICLES IN MODULATION OF PROTEIN EXPRESSION. ScholarBank@NUS Repository.
Abstract: Gold nanoparticles (AuNPs) allow ease of conjugation to thiol-modified biomolecules like DNA and peptide. However, formation of protein corona could undesirably mask conjugated biomolecules’ performance. In this thesis, we explored new ways of exploiting protein corona, in terms of complementary proteins recruitment to conjugated biomolecules on AuNPs’ surface for better biomolecular performance. We demonstrated that by using AuNPs functionalized with DNA (AuNP-DNA), we were able to exploit protein corona formation for ribosomes recruitment to enhance mRNA translation of single and multiple mRNA species in HeLa lysate. Similarly, there was sustained enhancement in insulin secretion when rat insulin producing cells were dosed with AuNP-DNA. Lastly, we expanded the breadth of this technology by exploiting protein corona formation around AuNPs functionalized with peptide (AuNP-peptide) to recruit MDM2/p53 complexes for improved p53 proteins stability. The outcome of this thesis will provide more insights on ways protein corona could be exploited in engineering design.
URI: http://scholarbank.nus.edu.sg/handle/10635/150683
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Chan Kian Ping Final Phd Thesis.pdf6.34 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

120
checked on Sep 25, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.