Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/150355
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dc.titleCATCHING MALARIA INFECTED RED BLOOD CELLS: A BIOPHYSICAL STUDY OF ICAM-1 MEDIATED CYTOADHESION USING IN VITRO METHODS
dc.contributor.authorLIM YING, BENA
dc.date.accessioned2018-12-31T18:01:36Z
dc.date.available2018-12-31T18:01:36Z
dc.date.issued2018-08-06
dc.identifier.citationLIM YING, BENA (2018-08-06). CATCHING MALARIA INFECTED RED BLOOD CELLS: A BIOPHYSICAL STUDY OF ICAM-1 MEDIATED CYTOADHESION USING IN VITRO METHODS. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/150355
dc.description.abstractPlasmodium (P.) falciparum malaria infected red blood cells (IRBCs) avoid splenic clearance by adhering to host endothelium. Upregulation of endothelial ICAM-1 and CD36 have been associated with severe disease pathology, but only ICAM-1 is highly expressed in the brain. This thesis aims to understand IRBC-ICAM-1 interactions from a biophysical perspective, using IRBC-CD36 bonds as a comparison. Single bond force spectroscopy was first conducted to study the nature of IRBC-ICAM-1 bonds and obtain bond dissociation parameters. Fever experienced during malaria infection subsequently motivated us to investigate the impact of temperature - 37°C (body) and 41°C (febrile) - on IRBC-ICAM-1 dissociation. Thereafter, we studied IRBC-ICAM-1 association. A cellular stochastic model was developed to indirectly extract bond association parameters from a flow assay using dissociation rates obtained earlier. Likewise, experiments were performed at 25°C, 37°C and 41°C. Results presented in this dissertation provide insight to IRBC-ICAM-1 adhesion kinetics and adjunctive treatment for malaria.
dc.language.isoen
dc.subjectICAM-1, malaria, catch bond, biophysics, Plasmodium falciparum, physiological temperature
dc.typeThesis
dc.contributor.departmentBIOMEDICAL ENGINEERING
dc.contributor.supervisorLIM CHWEE TECK
dc.contributor.supervisorJIANSHU CAO
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
Appears in Collections:Ph.D Theses (Open)

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