Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/150316
Title: IDENTIFICATION OF FUNCTIONAL PROTEINS INTERACTING WITH MMSET IN MULTIPLE MYELOMA
Authors: CHOOI JING YUAN
Keywords: Multiple myeloma, NSD2, chromosomal translocation t(4;14), SMARCA2
Issue Date: 23-Aug-2018
Citation: CHOOI JING YUAN (2018-08-23). IDENTIFICATION OF FUNCTIONAL PROTEINS INTERACTING WITH MMSET IN MULTIPLE MYELOMA. ScholarBank@NUS Repository.
Abstract: Recurrent chromosomal translocations are central to the pathogenesis of MM, with t(4;14) being the second-most common and associated with poor prognosis. NSD2 is overexpressed as a result of the t(4;14) translocation and has been suggested to be the primary oncogenic factor in t(4;14)+ MM. To address the relevant pathways that contributed to myelomagenesis, SILAC-based mass-spectrometry analysis was used to determine NSD2-interacting proteins. We identified 74 proteins and in silico analysis showed that one of them, SMARCA2, interacts with NSD2. Comparison of SMARCA2 expression across MM cell lines with different translocation statuses showed that SMARCA2 was highly expressed in t(4;14)+ MM cells but not in t(4;14)- MM cells. SMARCA2 KD in t(4;14)+ MM cells showed reduced cell growth and capacity to form colonies. We further investigated how NSD2 and SMARCA2 regulate the expressions of key myeloma genes, such as PRL3 and CCND1.
URI: http://scholarbank.nus.edu.sg/handle/10635/150316
Appears in Collections:Ph.D Theses (Restricted)

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