Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/150308
Title: CRL4 E3 UBIQUITIN LIGASE PROMOTES NEURAL STEM CELL REACTIVATION
Authors: LY PHUONG THAO
ORCID iD:   orcid.org/0000-0002-5986-2735
Keywords: Neural stem cells, quiescence, reactivation, E3 ubiquitin ligase, DCAF1, Hippo pathway
Issue Date: 1-Oct-2018
Citation: LY PHUONG THAO (2018-10-01). CRL4 E3 UBIQUITIN LIGASE PROMOTES NEURAL STEM CELL REACTIVATION. ScholarBank@NUS Repository.
Abstract: The ability of neural stem cells (NSCs) to transit between quiescence and proliferation is crucial for brain development and homeostasis. Drosophila Hippo pathway maintains NSC quiescence, but its regulation during brain development remains unknown. Here, we showed that CRL4Mahj, an evolutionarily conserved E3 ubiquitin ligase, is essential for NSC reactivation (exit from quiescence). We demonstrated that DDB1 and Cullin4, two core components of CRL4, are intrinsically required for NSC reactivation. We have identified a substrate receptor of CRL4, Mahjong (Mahj), which is necessary and sufficient for NSC reactivation. Moreover, we showed that CRL4Mahj forms a protein complex with Warts (Wts/Lats), a kinase of the Hippo signaling pathway, and Mahj promotes the ubiquitination of Wts. Our genetic analyses further support the conclusion that CRL4Mahj triggers NSC reactivation by inhibition of Wts. Given that Cullin4B mutations cause mental retardation and cerebral malformation, similar regulatory mechanisms may be applied to the human brain.
URI: http://scholarbank.nus.edu.sg/handle/10635/150308
Appears in Collections:Ph.D Theses (Restricted)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Thesis_Phuong Thao Ly_20181119 revised - No Appendix reduced[8717].pdf8.47 MBAdobe PDF

RESTRICTED

NoneLog In

Page view(s)

17
checked on Jul 10, 2020

Download(s)

6
checked on Jul 10, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.