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|Title:||UNRAVELING THE ROLE OF TRIM28 IN SPERMATOGENESIS||Authors:||TAN HENG LOONG JOEL||Keywords:||TRIM28, Spermatogenesis, Haploinsufficiency, Infertility, SSC||Issue Date:||7-Aug-2018||Citation:||TAN HENG LOONG JOEL (2018-08-07). UNRAVELING THE ROLE OF TRIM28 IN SPERMATOGENESIS. ScholarBank@NUS Repository.||Abstract:||Tripartite motif-containing 28 (TRIM28) is a prominent epigenetic transcriptional co-regulator that has been shown to regulate numerous biological processes such as cellular differentiation. However, little is understood about the role of TRIM28 in spermatogenesis except that ablating it leads to testicular degeneration in mice. In my laboratory, we observed that Trim28-heterozygous (Trim28Het) male mice became infertile prematurely, pointing to a likely haploinsufficiency phenotype of Trim28. Mating experiments confirmed this observation. As these mice grew older, their testes progressively shrank compared to the wild type, which likely resulted from the loss of germ cells. Halving the amount of TRIM28 in the germ cells phenocopied Trim28Het testes, indicating a germ cell autonomous effect. These heterozygous germ cells were progressively lost, beginning with the undifferentiated spermatogonia. Contrary to previous publication, I detected Trim28 expression in undifferentiated spermatogonia, including the spermatogonial stem cells (SSC). Based on these results, TRIM28 appears to regulate SSC function.||URI:||http://scholarbank.nus.edu.sg/handle/10635/149603|
|Appears in Collections:||Ph.D Theses (Open)|
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