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https://scholarbank.nus.edu.sg/handle/10635/149304
DC Field | Value | |
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dc.title | PTEN-L IS A NOVEL PROTEIN PHOSPHATASE FOR UBIQUITIN DEPHOSPHORYLATION TO INHIBIT PINK1-PARKIN-MEDIATED MITOPHAGY | |
dc.contributor.author | WANG LIMING | |
dc.date.accessioned | 2018-11-29T18:00:21Z | |
dc.date.available | 2018-11-29T18:00:21Z | |
dc.date.issued | 2018-08-17 | |
dc.identifier.citation | WANG LIMING (2018-08-17). PTEN-L IS A NOVEL PROTEIN PHOSPHATASE FOR UBIQUITIN DEPHOSPHORYLATION TO INHIBIT PINK1-PARKIN-MEDIATED MITOPHAGY. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/149304 | |
dc.description.abstract | Mitophagy, a selective form of autophagy for removal of damaged mitochondria and defective mitophagy, is well known to be implicated in the pathogenesis of neurodegenerative disorders, in particular Parkinson’s disease. PTEN-induced kinase protein 1 (PINK1)-mediated phosphorylation of ubiquitin plays a critical role in the onset of mitophagy. Phosphatase and tensin homolog (PTEN)-long (PTEN-L) is a newly identified isoform of PTEN, with addition of 173 amino acids to its N-terminal. In this study, we report a novel function of PTEN-L in the regulation of mitophagy. PTEN-L localizes on the outer mitochondrial membrane and inhibits Parkin mitochondrial translocation, its E3 ligase activity and phosphorylation induced by mitochondrial damaging agents. Moreover, PTEN-L reduces phospho-ubiquitin (pSer65-Ub) and in vitro phosphatase assay confirms that PTEN-L dephosphorylates ubiquitin via its protein phosphatase activity independent of its lipid phosphatase function. Thus, our findings demonstrate that PTEN-L is a novel protein phosphatase for ubiquitin in suppression of mitophagy. | |
dc.language.iso | en | |
dc.subject | mitophagy, phosphatase, ubiquitin, PTEN-L, Parkin, PINK1 | |
dc.type | Thesis | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.supervisor | Suen Mei,Celestial Therese Yap | |
dc.contributor.supervisor | Han Ming Shen | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY (SOM) | |
Appears in Collections: | Ph.D Theses (Open) |
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Wang LM.pdf | 6.21 MB | Adobe PDF | OPEN | None | View/Download |
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