Please use this identifier to cite or link to this item: https://doi.org/10.1006/jmcc.2002.2014
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dc.titlePhenylephrine promotes phosphorylation of bad in cardiac myocytes through the extracellular signal-regulated kinases 1/2 and protein kinase A
dc.contributor.authorValks D.M.
dc.contributor.authorCook S.A.
dc.contributor.authorPham F.H.
dc.contributor.authorMorrison P.R.
dc.contributor.authorClerk A.
dc.contributor.authorSugden P.H.
dc.date.accessioned2018-11-29T07:18:02Z
dc.date.available2018-11-29T07:18:02Z
dc.date.issued2002
dc.identifier.citationValks D.M., Cook S.A., Pham F.H., Morrison P.R., Clerk A., Sugden P.H. (2002). Phenylephrine promotes phosphorylation of bad in cardiac myocytes through the extracellular signal-regulated kinases 1/2 and protein kinase A. Journal of Molecular and Cellular Cardiology 34 (7) : 749-763. ScholarBank@NUS Repository. https://doi.org/10.1006/jmcc.2002.2014
dc.identifier.issn222828
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/149286
dc.description.abstractStudies in non-cardiomyocytic cells have shown that phosphorylation of the Bcl-2 family protein Bad on Ser-112. Ser-136 and Ser-155 decreases its pro-apoptotic activity. Both phenylephrine (100 �M) and the cell membrane-permeating cAMP analog, 8-(4-chlorophenylthio)-cAMP (100 �M), protected against 2-deoxy-D-glucose-induced apoptosis in neonatal rat cardiac myocytes as assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). In cardiac myocytes, phenylephrine primarily stimulates the ?-adrenoceptor, but, at high concentrations (100 �M), it also increases the activity of the cAMP-dependent protein kinase, protein kinase A (PKA) through the ?-adrenoceptor. Phenylephrine (100 �M) promoted rapid phosphorylation of Bad(Ser-112) and Bad(Ser-155), though we were unable to detect phosphorylation of Bad(Ser-136). Phosphorylation of Bad(Ser-112) was antagonized by either prazosin or propranolol, indicating that this phosphorylation required stimulation of both ?1- and ?-adrenoceptors. Phosphorylation of Bad(Ser-155) was antagonized only by propranolol and was thus mediated through the ?-adrenoceptor. Inhibitor studies and partial purification of candidate kinases by fast protein liquid chromatography showed that the p90 ribosomal S6 kinases, p90RSK2/3 [which are activated by the extracellular signal-regulated kinases 1 and 2 (ERK1/2)] directly phosphorylated Bad(Ser-112), whereas the PKA catalytic subunit directly phosphorylated Bad(Ser-155). However, efficient phosphorylation of Bad(Ser-112) also required PKA activity. These data suggest that, although p90RSK2/3 phosphorylate Bad(Ser-112) directly, phosphorylation of this site is enhanced by phosphorylation of Bad(Ser-155). These phosphorylations potentially diminish the pro-apoptotic activity of Bad and contribute to the cytoprotective effects of phenylephrine and 8-(4-chlorophenylthio)-cAMP. � 2002 Elsevier Science Ltd. All rights reserved.
dc.publisherAcademic Press
dc.sourceScopus
dc.subjectAdrenoceptors
dc.subjectApoptosis
dc.subjectBcl-2 proteins
dc.subjectcAMP
dc.subjectCardiac myocytes
dc.subjectMitogen-activated protein kinases
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1006/jmcc.2002.2014
dc.description.sourcetitleJournal of Molecular and Cellular Cardiology
dc.description.volume34
dc.description.issue7
dc.description.page749-763
dc.published.statepublished
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