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Title: Effect of intracellular cholesterol trafficking on its efflux to APOA-I
Authors: WEN CHI
Keywords: cholesterol trafficking, cholesterol efflux, apolipoprotein A-I, caveolae, MβCD, ABCA1
Issue Date: 6-Jun-2005
Citation: WEN CHI (2005-06-06). Effect of intracellular cholesterol trafficking on its efflux to APOA-I. ScholarBank@NUS Repository.
Abstract: HDL or its apolipoproteins play a key role in reverse cholesterol transport (RCT) which removes excess free cholesterol from cells to maintain cellular cholesterol homeostasis. This process could prevent the excessive cholesterol accumulation on the vessel wall and the development of atherosclerosis. The goal of this study is to investigate the mechanism of intracellular cholesterol trafficking and its effect on efflux to apolipoprotein A-I, the main protein of HDL particles, in human fibroblast. The results of this research indicated that microtubules, U18666A-inhibited cholesterol intracellular trafficking and ER-plasma membrane contacts do not affect cholesterol efflux at any significant level and Golgi apparatus probably plays a minor role in nascent cholesterol transport. However, disassembly of caveolae by cholesterol depletion or disruption of actin filaments both increased cholesterol efflux to apoA-I dramatically. It is therefore concluded that caveolae play a key regulatory role in both cholesterol intracellular trafficking and efflux.
Appears in Collections:Master's Theses (Open)

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