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Title: The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Keywords: hESCs, SAGE, transcriptome, xeno-free, cryopreservation, differentiation
Issue Date: 20-Sep-2005
Citation: RICHARDS MARK (2005-09-20). The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders. ScholarBank@NUS Repository.
Abstract: Human embryonic stem cells (hESCs) are derived from the isolation and serial sub-culture of inner cell masses from 5-day old human blastocysts. Immense commercial interest as well as ethical controversy surrounds hESC research. In principle, hESCs are capable of differentiating into all cell types in the adult human. Therefore they have enormous potential to provide a source of tissues for replacement in diseases in which native cell types are inactivated or destroyed. However, before the full therapeutic potential of hESC reparative cell therapy can be realized, a better understanding of fundamental hESC biology and hESC growth conditions is essential. In particular, improvements in culture techniques, the identification of genes that control hESC pluripotency and efficient directed differentiation strategies need to be developed before hESCs can become of any therapeutic use. The studies in this thesis address some urgent issues pertaining to hESC research, more specifically the derivation and propagation of xeno-free hESC lines, the xeno-free storage of hESCs and the understanding of the gene expression profiles of hESCs with serial analysis of gene expression (SAGE) that can help identify genes involved in maintaining the pluripotent phenotype as well as genes involved in differentiation events.
Appears in Collections:Ph.D Theses (Open)

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