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Title: | The hepatitis B core promoter: Control of viral life cycle events | Authors: | ONG SHAIR LING, CARMEN | Keywords: | HBV, core promoter, viral replication | Issue Date: | 25-May-2005 | Citation: | ONG SHAIR LING, CARMEN (2005-05-25). The hepatitis B core promoter: Control of viral life cycle events. ScholarBank@NUS Repository. | Abstract: | The HBV core promoter transcribes both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. Pairedsamples, pre- and post HBeAg seroconversion, were selected from 14 chronic HBVpatients who had a significant decrease in serum viral load after HBeAgseroconversion implying a drop in viral replication. The CP acitivty from each samplewas quantitated via a luciferase reporter gene. 5a?? RACE was also carried out todistinguish between pre-core and pre-genomic mRNA transcription. Sequenceanalysis showed the presence of previously reported and novel CP mutations whichcaused a change in CP activity. However, the correlation between CP activity andviral replication was not significant. Hence, we conclude that the regulation of HBVreplication is a complex process in which the CP plays a necessary, but not sufficient,role. | URI: | http://scholarbank.nus.edu.sg/handle/10635/14799 |
Appears in Collections: | Master's Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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01-Title Page.pdf | 6.61 kB | Adobe PDF | OPEN | None | View/Download | |
02-Table of Contents.pdf | 47.4 kB | Adobe PDF | OPEN | None | View/Download | |
03-Introduction.pdf | 218.55 kB | Adobe PDF | OPEN | None | View/Download | |
04-Methods.pdf | 437.28 kB | Adobe PDF | OPEN | None | View/Download | |
05-Results.pdf | 290.44 kB | Adobe PDF | OPEN | None | View/Download | |
06-Discussion.pdf | 63.64 kB | Adobe PDF | OPEN | None | View/Download | |
07-References.pdf | 49.91 kB | Adobe PDF | OPEN | None | View/Download | |
08-APPENDIX.pdf | 15.99 MB | Adobe PDF | OPEN | None | View/Download |
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