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|Title:||Identification of novel microRNAs in the sheep heart and their regulation in heart failure||Authors:||Wong L.L.
|Issue Date:||15-Aug-2017||Publisher:||Nature Publishing Group||Citation:||Wong L.L., Rademaker M.T., Saw E.L., Lew K.S., Ellmers L.J., Charles C.J., Richards A.M., Wang P. (2017-08-15). Identification of novel microRNAs in the sheep heart and their regulation in heart failure. Scientific Reports 7 (1) : 8250. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-08574-x||Abstract:||Study of microRNA (miRNAs) using sheep models is limited due to lack of miRNA information. We therefore investigated oar-miRNAs and their regulation in an ovine model of heart failure (HF). Left ventricular (LV) tissue was collected from normal (Cont), HF (LV pacing @ ~220bpm for 13-days) and HF-recovery sheep (HF-R, 26-days after pacing cessation). MiRNA expression was profiled using next-generation sequencing (NGS) and miRNA array, and validated by stem-loop qPCR. Detected sequences were mapped against the ovine genome (Oar v4.0) and aligned with known miRNAs (miRBase v21). A total of 36,438,340 raw reads were obtained with a peak distribution of 18-23 nt. Of these, 637 miRNAs were detected by NGS and mapped to the ovine genome. With cut-off at 10 counts, 275 novel miRNAs were identified (with 186 showing 100% alignment and 89 showing 70-99% alignment with human/mouse and/or rat miRNAs, respectively), and 78 known oar-miRNAs. Cardiac-enriched miRNA-1, -133a, -208a/b and -499 were highly expressed in the LV. With HF induction, miRNA-133b-3p, -208b-3p, -125a-5p, -125b-5p, -126-3p, -21-5p, -210-3p, -29a-3p, -320a and -494-3p were significantly up-regulated relative to Cont and tended to return to normal levels following HF-recovery. This study has expanded the sheep miRNA database, and demonstrated HF-induced regulation of miRNAs. � 2017 The Author(s).||Source Title:||Scientific Reports||URI:||http://scholarbank.nus.edu.sg/handle/10635/146671||ISSN:||20452322||DOI:||10.1038/s41598-017-08574-x|
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