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|Title:||GENETIC STUDIES TO UNDERSTAND OUTER MEMBRANE LIPID HOMEOSTASIS IN ESCHERICHIA COLI||Authors:||JIANG XIANG'ER||Keywords:||Tol-Pal Complex,Lipid homeostasis,enterobacterial common antigen (ECA),Phospholipids,Und-PP,Outer membrane||Issue Date:||28-Mar-2018||Citation:||JIANG XIANG'ER (2018-03-28). GENETIC STUDIES TO UNDERSTAND OUTER MEMBRANE LIPID HOMEOSTASIS IN ESCHERICHIA COLI. ScholarBank@NUS Repository.||Abstract:||Cells lacking the Tol-Pal complex accumulate excess phospholipids (PLs) in the OM due to defective retrograde transport of PLs from the outer membrane (OM) back to the inner membrane (IM). We aim to understand the role of the Tol-Pal complex in maintaining OM lipid homeostasis via genetic studies. We first restored lipid ratios in the OM of tol-pal mutants by adjusting the levels of LpxC and FabZ. Meanwhile, we selected for genetic suppressors that can rescue sensitivity of tol-pal mutants to vancomycin. We showed that this rescue phenotype is dependent on the accumulation of enterobacterial common antigen (ECA) biosynthetic intermediates in the cell, and yet not related to cell wall stress due to undecaprenyl phosphate (und-P) sequestration. The rescue phenotype is also independent of activation of the Rcs phosphorelay stress response pathway. Further insights into how ECA intermediates suppress vancomycin sensitivity in tol-pal strains will be presented.||URI:||http://scholarbank.nus.edu.sg/handle/10635/146464|
|Appears in Collections:||Ph.D Theses (Open)|
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