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Title: Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (Lates calcarifer)
Keywords: seabass, testicular cell culture, sex differentiation , gonadogenesis
Issue Date: 29-Mar-2005
Citation: LIM MENG HUAT (2005-03-29). Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (Lates calcarifer). ScholarBank@NUS Repository.
Abstract: Genes vasa, dazl and sox3 are involved in gonadal development and sex differentiation. The marine seabass is an ideal model for studying these processes because it matures as male and undergoes spontaneous sex reversal to female. This thesis is aimed at making use of this unique organism to study germ cell development and sex differentiation. cDNAs for the above 3 genes were isolated and their RNA expression patterns analysed during embryonic and adult development. Conditions for isolating and culturing seabass testicular cells as an in-vitro model were established. Adult testicular cells can be cultured for up to 40 days. Vasa, dazl and sox3 were transcribed during the culture period, indicating that the cell population comprised of germ cells. These results demonstrate that the cDNAs cloned encode the seabass orthologs for vasa, dazl and sox3, which are all expressed in the gonads. They will provide a useful tool for ongoing experiments to study gonadogenesis and sex differentiation in the seabass as an unique vertebrate model.
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