Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/145042
Title: CHARACTERIZING A NOVEL EFFECTOR PROTEIN OF EDWARDSIELLA TARDA (E. TARDA): THIOREDOXINLIKE PROTEIN (TRX3)
Authors: AHMED MAHMOUD MOHAMMED SAYED
Keywords: E. tarda, TRX3, PRX, Redox activity, Cysteine reactivity, NF-κB
Issue Date: 11-Apr-2018
Citation: AHMED MAHMOUD MOHAMMED SAYED (2018-04-11). CHARACTERIZING A NOVEL EFFECTOR PROTEIN OF EDWARDSIELLA TARDA (E. TARDA): THIOREDOXINLIKE PROTEIN (TRX3). ScholarBank@NUS Repository.
Abstract: Edwardsiella tarda (E. tarda) is a gram-negative bacteria which infects fish, birds and humans. It causes a great loss to the fishing industry every year. E. tarda exhibits its virulence by secreting its effector proteins into the host cell. Recent transcriptomic and bioinformatic analysis have identified novel effector proteins of E. tarda. One of the effectors, ETAE_2186, is identified as a Thioredoxin-Like Protein (TRX3). We have characterized the structure of TRX3 and elucidated substantial differences in the active site “CPVC” as compared to other TRXs. In addition, based on our cellular and biochemical assays, we have characterized TRX3 as a redox-inactive protein, which can inhibit NF-κB translocation in the host. Inhibition of NF-κB translocation is known to further downregulate the anti-inflammatory genes. Finally, we suggest a model to describe TRX3 role in NF-κB inhibition pathway during E. tarda pathogenesis.
URI: http://scholarbank.nus.edu.sg/handle/10635/145042
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Sayed AMM.pdf15.37 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

85
checked on Oct 16, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.