Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/144701
Title: TARGETED TRANSGENE INSERTION INTO THE AAVS1 LOCUS VIA ZINC FINGER NUCLEASE TECHNOLOGY FOR CANCER IMMUNOTHERAPY
Authors: TAY CHANG FELIX
Keywords: Gene editing, zinc finger nuclease, AAVS1, induced pluripotent stem cells, cancer stem cells, cancer immunotherapy
Issue Date: 21-Jan-2016
Citation: TAY CHANG FELIX (2016-01-21). TARGETED TRANSGENE INSERTION INTO THE AAVS1 LOCUS VIA ZINC FINGER NUCLEASE TECHNOLOGY FOR CANCER IMMUNOTHERAPY. ScholarBank@NUS Repository.
Abstract: IN THIS STUDY, WE DEMONSTRATE HOW ZINC FINGER NUCLEASE (ZFNS) CAN BE UTILIZED FOR THE ADVANCEMENT IN CANCER IMMUNOTHERAPY. FIRSTLY, A BACULOVIRAL VECTOR-MEDIATED ZINC FINGER NUCLEASE (BV-ZFN) TECHNOLOGY WAS DEVELOPED. IT DEMONSTRATED A HIGH TARGETING EFFICIENCY AND MAINTAINED EXPRESSION STABILITY OF THE TRANSGENE INSERTED INTO THE AAVS1 LOCUS OF HUMAN INDUCED PLURIPOTENT STEM CELLS (HIPSCS). NEXT, USING THE BV-ZFN TECHNOLOGY, HUMAN FIBROBLASTS WERE EFFICIENTLY REPROGRAMMED INTO HIPSCS BY INTEGRATING 4 REPROGRAMMING GENES INTO THE AAVS1 LOCUS. THE SAME REPROGRAMMING TECHNIQUE WAS USED TO REPROGRAM HEPATOCELLULAR CARCINOMA (HCC) CELLS INTO CANCER STEM CELL (CSC)-LIKE CELLS THAT EXHIBITED CLASSICAL CSC CHARACTERISTICS. THESE CSC-LIKE CELLS COULD BE USED AS AN INFINITE SOURCE OF CSC-ASSOCIATED ANTIGENS FOR THE GENERATION OF DENDRITIC CELL (DC)-BASED VACCINE AGAINST HCC CSC. LASTLY, IMPROVEMENTS IN THE EXPANSION OF NATURAL KILLER (NK) CELL WERE ACHIEVED BY UTILIZING ZFNS TO INTRODUCE MEMBRA
URI: http://scholarbank.nus.edu.sg/handle/10635/144701
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TayCF.pdf6.24 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

104
checked on May 29, 2020

Download(s)

37
checked on May 29, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.