Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/142062
Title: A NOVEL STEM-CELL BASED CO-CULTURE DEVICE TO STUDY NEUROMUSCULAR DISORDERS
Authors: PLUCHON PERRINE YVONNE JACQUELINE
Keywords: Neuromuscular junctions, neuromuscular disorders, ALS, stem cells, microfabricated devices, optogenetics
Issue Date: 10-Jan-2018
Citation: PLUCHON PERRINE YVONNE JACQUELINE (2018-01-10). A NOVEL STEM-CELL BASED CO-CULTURE DEVICE TO STUDY NEUROMUSCULAR DISORDERS. ScholarBank@NUS Repository.
Abstract: Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease characterised by the degeneration of neuromuscular junctions and motor neurons. To gain insight into mechanisms of ALS and other neuromuscular disorders, it is crucial to develop a relevant in vitro system to model nerve-muscle connectivity. We established a compartmentalised model to co-culture motor neurons, astrocytes and muscle. Individual neural cell types were derived from mouse embryonic stem cells and purified while muscle was derived from chick embryonic primary myoblasts. The cells were assembled in microfabricated devices that segregate the cell bodies of neural cells from muscle fibres to mimic the normal anatomy of neuromuscular connectivity. Motor neurons were equipped with Channelrhodopsin-2, enabling their selective optogenetic activation. Using this device, we have achieved long-term co-cultures of muscle and neural tissues. We have also demonstrated the formation of neuromuscular junctions and their functionality.
URI: http://scholarbank.nus.edu.sg/handle/10635/142062
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
02_PluchonP.pdf27.97 MBAdobe PDF

OPEN

NoneView/Download
03_PluchonP.pdf6.51 MBAdobe PDF

OPEN

NoneView/Download
01_PluchonP.pdf28.68 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

59
checked on Jul 10, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.