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Title: Ontogeny and hormonal regulation of alpha-amylase gene expression in seabass larvae, Lates calcarifer
Keywords: alpha-amylase, seabass, promoter, AR42-J cell, glucocorticoid response element, thyroid
Issue Date: 1-Jun-2004
Citation: MA PEISONG (2004-06-01). Ontogeny and hormonal regulation of alpha-amylase gene expression in seabass larvae, Lates calcarifer. ScholarBank@NUS Repository.
Abstract: To understand the development of digestive functions in marine fish larvae, the ontogeny and regulation of gene expression of alpha-amylase were studied in seabass larvae (Lates calcarifer). A correlation between enzymatic activity and mRNA level of alpha-amylase was demonstrated. This suggests that the changes in alpha-amylase are controlled at the transcriptional level.A glucocorticoid response element (GRE) was identified on seabass amylase promoter, but, no functional PTF-1 binding site was identified. Instead a functional HNF-3 binding site was found.Dexamethasone induced GRE activity in vitro and cortisol treatment of seabass larvae upregulated amylase gene expression. Thyroid hormone (T3) treatment also upregulated the gene expression and no T3 response element was identified on the promoter, suggesting indirect action of T3. Food restriction and deprivation upregulated amylase gene expression. The findings are interpreted as a stress response whereby cortisol secretion was elevated which activated the GR-transcription system and upregulated amylase gene expression.
Appears in Collections:Ph.D Theses (Open)

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